课题组名称 疾病标志物超灵敏检测

研究组长介绍

邮箱：xu.wang@shsmu.edu.cn

王旭，博士，本科和硕士毕业于大连理工大学，师从于生物化学专家彭孝军院士。博士毕业于德国慕尼黑工业大学，师从于欧洲著名分析化学家Reinhard Niessner教授。2017年到2021年为美国哈佛大学助理研究员，合作导师为美国三院院士、Illumina创始人 David R. Walt 教授。王旭博士在 JACS、Chemical Science 等著名刊物发表多篇一作论文，并申请多项国际专利，担任Frontiers in Chemistry客座副主编。

课题组致力于超灵敏检测技术的开发，检测蛋白质、酶、核酸、小分子等疾病标志物，并搭建配套的检测仪器，用于癌症、心血管疾病、神经退行性疾病的早检和诊断。

主要研究方向如下：

1.开发超灵敏、高通量的单分子免疫分析检测疾病标志物

2.开发超灵敏的方法检测酶活性，并用于单细胞分析和药物筛选

3.开发新型荧光检测方法，用于细胞和组织内的蛋白质、酶、RNA、活性氧等的成像

代表性论文：

1.Xu Wang, Alana F. Ogata and David R. Walt. Ultrasensitive Detection of Enzymatic Activity Using Single Molecule Arrays. J. Am. Chem. Soc. 2020, 142, 15098–15106.

2.Xu Wang and David R. Walt. Simultaneous Detection of Small Molecules, Proteins and microRNAs Using Single Molecule Arrays. Chem. Sci. 2020, 11, 7896-7903.

3.Xu Wang, Limor Cohen, Jun Wang and David R. Walt. Competitive Immunoassays for the Detection of Small Molecules Using Single Molecule Arrays. J. Am. Chem. Soc. 2018, 140, 18132-18139.

4.Xu Wang, Feng Li and Yirong Guo. Recent Trends in Nanomaterial-Based Biosensors for Point-of-Care Testing. Front. Chem. 2020, DOI: 10.3389/fchem.2020.586702.

5.Xu Wang, Reinhard Niessner, Dianping Tang and Dietmar Knopp*. Nanoparticle-Based Immunosensors and Immunoassays for Aflatoxins. Analytica Chimica Acta, 2016, 912, 10-23.

6.Xu Wang, Fengling Song,* and Xiaojun Peng. A Versatile Fluorescent Probe for Imaging Viscosity and Hypochlorite in Living Cells. Dyes and Pigments, 2016, 125, 89-94.

7.Xu Wang, Jutta Pauli, Reinhard Niessner, Ute Resch-Genger and Dietmar Knopp*. Gold Nanoparticle-Catalyzed Uranine Reduction for Signal Amplification in Fluorescent Assays for Melamine and Aflatoxin B1. Analyst, 2015, 140, 7305-7312.

8.Xu Wang, Reinhard Niessner and Dietmar Knopp*. Controlled Growth of Immunogold for Amplified Optical Detection of Aflatoxin B1. Analyst, 2015, 140, 1453-1458.

9.Xu Wang, Reinhard Niessner and Dietmar Knopp*. Magnetic Bead-Based Colorimetric Immunoassay for Aflatoxin B1 Using Gold Nanoparticles. Sensors, 2014, 14, 21535-21548.

团队成员：

张新潮，博士，助理研究员

张悦，研究生

课题组长期招聘研究生和博士后等，欢迎咨询！