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Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.P &* Clinical information(j Patients were categorised into two groups according to the ASA score (<2 and >2) [4]. Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria [14]. 'ResultsClinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP'PPSPPPP'S (Clinical data  )LAccessory regulatory gene (agr) groups'& 3enterotoxins * Clonal complexes+MSCRAMM genes 68    ,Capsule types -$Virulence factors 2."Resistance genes /Discussion 0I#(  / 0PDArialD[SO DWingdings0DCalibri@DўSOPD_oŖў`DArial Unicode MSpDHaettenschweilerDMistralDLathaDeck|ў{SODTimes New RomanDMS PGothicDRockwellDeck|[{SODVerdanaDArial BlackDwiSO_GB2312 DNSe~ў0DMS UI Gothic@DNSewiSOPDeckY~{SO`DSegoe PrintpDSymbolDTrebuchet MSDSegoe PrintDSegoe PrintDe[SO@  @h" dd@o  @@`` dj     ?R$tm/"@4b R$&) 茼+dxz "$ G=;wF#i:s * fV+f3ff3̙@?  O  8/+ppp@ 80___PPT10 pp #Background DStaphylococcus aureus is a leading cause of surgical site infections (SSIs) [1], which are found in more than 50% of infections related to total joint arthroplasty [2]. However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI [3].DyoF     $Background The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large p1" @8 __WPP6pOp   ` 33` Sf3f` 33g` f` www3PP` ZXdbmo` \ғ3y`Ӣ` 3f3ff` 3f3FKf` hk]wwwfܹ` ff>>\`Y{ff` R>&- {p_/̴0" d@o,n" d@o   " @ ` `" d@o   @@``PR    @ ` ` p>> jb(    c <  Rectangle 2" `}  :USQdkYkHrh7h_   S 6  Rectangle 3" `  r8USQdkYkHre,g7h_ ,{N~ ,{ N~ ,{V~ ,{N~   S 6  Rectangle 4"^ `  T     S 6  Rectangle 5"^   V     S 6  Rectangle 6"^ `  v*"   \  6 A?"@` 33 mŖIS  ` 33` Sf3f` 33g` f` www3PP` ZXdbmo` \ғ3y`Ӣ` 3f3ff` 3f3FKf` hk]wwwfܹ` ff>>\`Y{ff` R>&- {p_/̴0" d@o,n" d@o   " @ ` `" d@o   @@``PR    @ ` ` p>> H@ (    0  " `}  :USQdkYkHrh7h_   s *  " `  r8USQdkYkHre,g7h_ ,{N~ ,{ N~ ,{V~ ,{N~   s *  "^ `  l*    s *  "^   n*     s *  "^ `  n*   N  6 ? 33  ؞!jg vn0 (     H  ?Rectangle 2P    R     H  ?Rectangle 3    *        N  ?Rectangle 4    N  ?Rectangle 5 0  8USQdkYkHre,g7h_ ,{N~ ,{ N~ ,{V~ ,{N~    N  ?Rectangle 6_P   R     N  ?Rectangle 7_   *      N   6 ? 33 ld@(    c $   b Molecular characterisation of methicillin-sensitive Staphylococcus aureus from deep surgical site infections in orthopaedic patients<4R4C RC V  0 A?"@` 33 P~(    H  Rectangle 2     s B  Rectangle 3 0   ,,g!jgegnNQ~ 1u,{NNQtetS^ MQ9RN~'Y[O(u0 fY|i_PPT!jg http://www.1kejian.com O(ue RddkYlsSS0 MreHhO9e MreHh(W00{^opGr0-->0MreHh0-->0MreHh0 Nte0 LOGOvmR LogomRO9e(W0ƉV0-->0kHr0-->0{^opGrkHr0 Nte0vc blogoVGr RdbO9e0 W[SO0kHr0-->0{^opGrkHr0 Nte0   $    ,   3   &     #                                      0 C  ? 33  ph`(  l  C   `}   l  C   `  0 C  ? 33  php(  l  C   `}   l  C   `  0 C  ? 33  ph (   l  C   `}   l  C   `  0  C  ? 33  ph$(  $l $ C   `}   l $ C   `  0 $C  ? 33  ph((  (l ( C   `}   l ( C   `  0 (C  ? 33  vn,(  ,l , C   `}   r , S   `  0 ,C  ? 33  vn0(  0l 0 C   `}   r 0 S   `  0 0C  ? 33  vn4(  4l 4 C   `}   r 4 S   `  0 4C  ? 33  vn8(  8l 8 C   `}   r 8 S   `  0 8C  ? 33  vn<(  <l < C  &fW   r < S   `  0 <C  ? 33  vn@(  @l @ C  Z9*   r @ S   `  0 @C  ? 33  vnD(  Dl D C  &fW   r D S   `  0 DC  ? 33  |t H$(  Hr H S   `}   r H S   `  0 HC  ? 33  0Lr(  Ll L C  Z9*   r L S   `  L L c $A 0? Tu0 LC  ? 33  vn@P(  Pl P C   `}   r P S   `  0 PC  ? 33  |tPT$(  Tr T S   `}   r T S   `  0 TC  ? 33  |t`X$(  Xr X S   `}   r X S   `  0 XC  ? 33J   p\ (  \ \ C   m q   FReferences P  \ c $ -D@ h Z 1.Anderson DJ, Kaye KS (2009) Staphylococcal surgical site infections. Infect Dis Clin Norlh Am 23:53-72. doi:10.1016/j.idc.2008.10.004 2.Rao N, Cannella BA, Crossett LS, Yates AJ Jr, McGough RL 3rd, Hamilton CW (2011) Preoperative screening/decolonization for Staphylococcus aureus to prevent orthopedic surgical site infection: prospective cohort study with 2-year follow-up. J Arthroplasty 26:1501-1507. doi:10.1016/j.arth.2011.03.014 3.Berthelot P, Grattard F, Cazorla C, Passot JP, Fayard JP, Meley R, Bejuy J, Farizon F, Pozzetto B, Lucht F (2010) Is nasal carriage of Staphylococcus aureus the main acquisition pathway for surgical- site infection in orthopaedic surgery? Eur J Clin Microbiol Infect Dis 29:373-382. doi:10.1007/s10096-009-0867-5 4.Dale H, Skramm I, L0wer HL, Eriksen HM, Espehaug B, Furnes O, Skjeldestad FE, Havelin LI, Engesaeter LB (2011) Infection after primary hip arthroplasty: a comparison of 3 Norwegian health registers. Acta Orthop 82:646-654. doi:10.3109/ 17453674.2011.636671 5.Saadatian-Elahi M, Teyssou R, Vanhems P (2008) Staphylococcus aureus, the major pathogen in orthopaedic and cardiac surgical site infections: a literature review. Int J Surg 6:238-245. doi:10.1016/j. ijsu.2007.05.001 6.Wertheim HF, Melles DC, Vos MC, van Leeuwen W, Van Belkum A, Verbrugh HA, Nouwen JL (2005) The role ofnasal carriage in Staphylococcus aureus infections. 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O  # Background  DStaphylococcus aureus is a leading cause of surgical site infections (SSIs) [1], which are found in more than 50% of infections related to total joint arthroplasty [2]. However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI [3].F     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P  %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.P   & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) [4]. Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria [14].   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O  # Background  DStaphylococcus aureus is a leading cause of surgical site infections (SSIs) [1], which are found in more than 50% of infections related to total joint arthroplasty [2]. However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI [3].F     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P  %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.P   & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) [4]. Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria [14].   'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data   )&Accessory regulatory gene (agr) groups '   3 enterotoxins   *:-hemolysin converting phages   +MSCRAMM genes .8     ,Capsule types    2Antibiotic resistance   -Virulence factors    .Resistance genes    / Discussion   0  1  "  /@8 __WPP6pOp   ,0(  ,r , S ,* 9  * ~ , >A table1 B ,s * ޽h? 33r (e`(  / 0PDArialPrinPP+ з0зB 0ȷ"D[SOalPrinPP+ з0зB 0ȷ DWingdingsPP+ з0зB 0ȷ0DCalibrisPP+ з0зB 0ȷ"@DўSOibrisPP+ з0зB 0ȷ1PD_oŖўrisPP+ wxyz w!1AQaq"2B #3Rbr $4%&'()*56789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz ? 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O  # Background  DStaphylococcus aureus is a leading cause of surgical site infections (SSIs) [1], which are found in more than 50% of infections related to total joint arthroplasty [2]. However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI [3].F     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P  %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.P   & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) [4]. Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria [14].   'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '   3 enterotoxins   *:-hemolysin converting phages   +MSCRAMM genes .8     ,Capsule types    2Antibiotic resistance   -Virulence factors    .Resistance genes    / Discussion   0  1  "  /@8 __WPP6pOp #  l#(  lr l S 'g    l  A ?  .   "image1VGr 2image1TTTT#" j\  a l 6L{f > Fig. 1 SplitsTree similarity plot showing the clusters of the clonal complexes (CCs)/sequence types (STs) constructed from the DNA microarray hybridisation results of 117 Staphylococcus aureus isolates taken from patients undergoing major orthopaedic surgery. The isolates from deep surgical site infections (SSIs) are represented by yellow boxes, whereas the isolates from nasal carriers are represented by green boxes   H l 0޽h ? 3380___PPT10.U7`` r4e#!(  / 0PDArialPrinPP+-з0зB 0зB 0ȷ DWingdingsPP+-з0зB 0ȷ0DCalibrisPP+-з0зB 0ȷ"@DўSOibrisPP+-з0зB 0ȷ1PD_oŖўrisPP+-з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweile"D[SOalPrinPP+-з0зB 0ȷ DWingdingsPP+-з0зB 0ȷ0DCalibrisPP+-з0зB 0ȷ"@DўSOibrisPP+-з0зB 0ȷ1PD_oŖўrisPP+-з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` jbpn     R$tm/"@4b R$&) 茼+dxz "$ G=;wF#i:b$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  # Background  DStaphylococcus aureus is a leading cause of surgical site infections (SSIs) [1], which are found in more than 50% of infections related to total joint arthroplasty [2]. However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI [3].F     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P  %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.P   & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) [4]. Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria [14].   'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '   3 enterotoxins   *:-hemolysin converting phages   +MSCRAMM genes .8     ,Capsule types    2Antibiotic resistance   -Virulence factors   The enterotoxin gene cluster egc (seg, sei, sem, sen, seo, seu),which were found in 47 (82.5%) of the nasal carriers and 41(68.3%) of the SSI isolates. In three nose carrier isolates and four deep SSI isolates, partial deletions of the egc cluster were observed. The egc cluster was present in 12 of 13 (92.3%) isolates from deep SSI with persistent infection and in 25 of 41 (61.0%) isolates from patients with successful eradication of their SSI.Z  .Resistance genes    / Discussion   0  1  "  /@8 __WPP6pOp   D*(  Dr D S " &fW  " x D c $"  ` " B Ds * ޽h? 33r_L -e;8n!(  / 0PDArialPrinPP+-з j  0зB 0ȷ"D[SOalPrinPP+-з0зB 0ȷ DWingdingsPP+-з0зB 0ȷ0DCalibrisPP+-з0зB 0ȷ"@DўSOibrisPP+-з0зB 0ȷ1PD_oŖўrisPP+-з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` jbpi     R$tm/"@4b R$&) 茼+dxz $b$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  # Background  DStaphylococcus aureus is a leading cause of surgical site infections (SSIs) [1], which are found in more than 50% of infections related to total joint arthroplasty [2]. However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI [3].F     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P  %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.P   & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) [4]. Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria [14].   'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '   3 enterotoxins   *:-hemolysin converting phages   +MSCRAMM genes .8     ,Capsule types    2Antibiotic resistance   -Virulence factors   The enterotoxin gene cluster egc (seg, sei, sem, sen, seo, seu),which were found in 47 (82.5%) of the nasal carriers and 41(68.3%) of the SSI isolates. In three nose carrier isolates and four deep SSI isolates, partial deletions of the egc cluster were observed. The egc cluster was present in 12 of 13 (92.3%) isolates from deep SSI with persistent infection and in 25 of 41 (61.0%) isolates from patients with successful eradication of their SSI.Z  / Discussion   0  1  "  /@8 __WPP6pOp rt/eP0R!(  / 0PDArialPrinPP+-з0зB 0ȷ"D[SOalPrinPP+-з0r0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` jbpe     R$tm/"@4b R$&) 茼+dxz $b$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  q# Background  DStaphylococcus aureus is a leading cause of surgical site infections (SSIs) [1], which are found in more than 50% of infections related to total joint arthroplasty [2]. However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI [3].F     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P  %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.P   & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) [4]. Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria [14].   'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '   3 enterotoxins   *:-hemolysin converting phages   +MSCRAMM genes .8     ,Capsule types    2Antibiotic resistance   -Virulence factors   The enterotoxin gene cluster egc (seg, sei, sem, sen, seo, seu),which were found in 47 (82.5%) of the nasal carriers and 41(68.3%) of the SSI isolates. In three nose carrier isolates and four deep SSI isolates, partial deletions of the egc cluster were observed. The egc cluster was present in 12 of 13 (92.3%) isolates from deep SSI with persistent infection and in 25 of 41 (61.0%) isolates from patients with successful eradication of their SSI.Z  / Discussion   0  1  "  /@8 __WPP6pOp r11e0xR&(  / 0PDArialPrinPP+=з0зB 0ȷ"D[SOalPrinPP+=з0зB 0ȷ DWingdingsPP+=з0зB 0ȷ0DCalibrisPP+=з0зB 0ȷ"@DўSOibrisPP+=з0зB 0ȷ1PD_oŖўrisPP+=з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` jbpe     R$tm/"@4b R$&) 茼+dxz $b$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  # Background  DStaphylococcus aureus is a leading cause of surgical site infections (SSIs) [1], which are found in more than 50% of infections related to total joint arthroplasty [2]. However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI [3].F     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P  %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.P   & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) [4]. Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria [14].   'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '   3 enterotoxins   *:-hemolysin converting phages   +MSCRAMM genes .8     ,Capsule types    2Antibiotic resistance   -Virulence factors   The enterotoxin gene cluster egc (seg, sei, sem, sen, seo, seu),which were found in 47 (82.5%) of the nasal carriers and 41(68.3%) of the SSI isolates. In three nose carrier isolates and four deep SSI isolates, partial deletions of the egc cluster were observed. The egc cluster was present in 12 of 13 (92.3%) isolates from deep SSI with persistent infection and in 25 of 41 (61.0%) isolates from patients with successful eradication of their SSI.Z  / Discussion  The MSCRAMM gene bbp was more frequent in deep SSI than in nasal carrier isolates, suggesting a role in S. aureus invasiveness in deep SSI. Bbp belongs to the adhesins, together with fibronectin-binding proteins A and B (FnbpA/B), clumping factors A and B (ClfA/B) and collagen adhesion (Cna). These adhesins have been suggested to mediate S. aureus adhesion on indwelling devices. VW"  0 Discussion   The agr system regulates the production of a diverse range of genes involved in metabolism, virulence, transport and degradation pathways, including the expression of adhesins and the subsequent formation of biofilm . Agr, thereby, plays an important role in S. aureus pathogenesis.V)  1 Discussion   Staphylokinase facilitate bacterial penetration into sur-rounding tissues and is claimed to be an important colonising factor . The presence of sak might be linked to higher invasiveness of S. aureus, finding the gene more frequently in patients than in asymptomatic carriers. @.O8    "  /@8 __WPP6pOp   @P*(  Pr P S $  `}  $ x P c $|$ zT $ B Ps * ޽h? 33  PT0(  Tx T c $$  `}  $ x T c $\$ T $ B Ts * ޽h? 33  `X0(  Xx X c $  `}   x X c $    B Xs * ޽h? 33 LD`(  ` ` BD #" `   D   ` 6 0)j rThank you  &P   B `s * ޽h? 33r R0[yG{9}+"eRρ&'(  / 0PDArialPrinPP+=з0зB 0ȷ"D[SOalPrinPP+=з0зB 0ȷ DWingdingsPP+=з0зB 0ȷ0DCalibrisPP+=з0зB 0ȷ"@DўSOibrisPP+=з0зB 0ȷ1PD_oŖўrisPP+=з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` jbpe     R$tm/"@4b R$&) 茼+dxz $b$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  E# Background  4Staphylococcus aureus is a leading cause of surgical site infections (SSIs) , which are found in more than 50% of infections related to total joint arthroplasty . However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI .Zb  p     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P&   %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.P@z       & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) [4]. Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria [14].   'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '   3 enterotoxins   *:-hemolysin converting phages   +MSCRAMM genes .8     ,Capsule types    2Antibiotic resistance   -Virulence factors   The enterotoxin gene cluster egc (seg, sei, sem, sen, seo, seu),which were found in 47 (82.5%) of the nasal carriers and 41(68.3%) of the SSI isolates. In three nose carrier isolates and four deep SSI isolates, partial deletions of the egc cluster were observed. The egc cluster was present in 12 of 13 (92.3%) isolates from deep SSI with persistent infection and in 25 of 41 (61.0%) isolates from patients with successful eradication of their SSI.Z  / Discussion  The MSCRAMM gene bbp was more frequent in deep SSI than in nasal carrier isolates, suggesting a role in S. aureus invasiveness in deep SSI. Bbp belongs to the adhesins, together with fibronectin-binding proteins A and B (FnbpA/B), clumping factors A and B (ClfA/B) and collagen adhesion (Cna). These adhesins have been suggested to mediate S. aureus adhesion on indwelling devices. VW"  0 Discussion   The agr system regulates the production of a diverse range of genes involved in metabolism, virulence, transport and degradation pathways, including the expression of adhesins and the subsequent formation of biofilm . Agr, thereby, plays an important role in S. aureus pathogenesis.V)  1 Discussion   Staphylokinase facilitate bacterial penetration into sur-rounding tissues and is claimed to be an important colonising factor . The presence of sak might be linked to higher invasiveness of S. aureus, finding the gene more frequently in patients than in asymptomatic carriers. @.O8    "  /@8 __WPP6pOp   `$(  r  S   `}   r  S  %  B s * ޽h? 33  p$(  r  S P 'gt   r  S  l  B s * ޽h? 33   $(   r  S   `}   r  S   `  B  s * ޽h? 33r0I/%eQ'(  / 0PDArialPrinPP+=з0зB 0ȷ"D[SOalPrinPP+=з0зB 0ȷ DWingdingsPP+=з0зB 0ȷ0DCalibrisPP+=з0зB 0ȷ"@DўSOibrisPP+=з0зB 0ȷ1PD_oŖўrisPP+=з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` jbpe     R$tm/"@4b R$&) 茼+dxz $b$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  p# Background  4Staphylococcus aureus is a leading cause of surgical site infections (SSIs) , which are found in more than 50% of infections related to total joint arthroplasty . However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI .Zb  p     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P&   %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.@z      & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) . Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria .L Q  Z    'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '   3 enterotoxins   *:-hemolysin converting phages   +MSCRAMM genes .8     ,Capsule types    2Antibiotic resistance   -Virulence factors   The enterotoxin gene cluster egc (seg, sei, sem, sen, seo, seu),which were found in 47 (82.5%) of the nasal carriers and 41(68.3%) of the SSI isolates. In three nose carrier isolates and four deep SSI isolates, partial deletions of the egc cluster were observed. The egc cluster was present in 12 of 13 (92.3%) isolates from deep SSI with persistent infection and in 25 of 41 (61.0%) isolates from patients with successful eradication of their SSI.Z  / Discussion  The MSCRAMM gene bbp was more frequent in deep SSI than in nasal carrier isolates, suggesting a role in S. aureus invasiveness in deep SSI. Bbp belongs to the adhesins, together with fibronectin-binding proteins A and B (FnbpA/B), clumping factors A and B (ClfA/B) and collagen adhesion (Cna). These adhesins have been suggested to mediate S. aureus adhesion on indwelling devices. VW"  0 Discussion   The agr system regulates the production of a diverse range of genes involved in metabolism, virulence, transport and degradation pathways, including the expression of adhesins and the subsequent formation of biofilm . Agr, thereby, plays an important role in S. aureus pathogenesis.V)  1 Discussion   Staphylokinase facilitate bacterial penetration into sur-rounding tissues and is claimed to be an important colonising factor . The presence of sak might be linked to higher invasiveness of S. aureus, finding the gene more frequently in patients than in asymptomatic carriers. @.O8    "  /@8 __WPP6pOp    $(   r  S  'gt   r  S    B  s * ޽h? 33  $$(  $r $ S  $  `}  $ r $ S  $ T3 $ B $s * ޽h? 33r? ~)eda'(  / 0PDArialPrinPP+=з0зB 0ȷ"D[SOalPrinPP+=з0зB 0ȷ DWingdingsPP+=з0зB 0ȷ0DCalibrisPP+=з0зB 0ȷ"@DўSOibrisPP+=з0зB 0ȷ1PD_oŖўrisPP+=з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` zr xn      R$tm/"@4b R$&) 茼+dxz $b$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  p# Background  4Staphylococcus aureus is a leading cause of surgical site infections (SSIs) , which are found in more than 50% of infections related to total joint arthroplasty . However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI .Zb  p     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P&   %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.@z      & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) . Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria .L Q  Z    'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '   3 enterotoxins   *:-hemolysin converting phages   +MSCRAMM genes .8     ,Capsule types    2Antibiotic resistance   -Virulence factors   The enterotoxin gene cluster egc (seg, sei, sem, sen, seo, seu),which were found in 47 (82.5%) of the nasal carriers and 41(68.3%) of the SSI isolates. In three nose carrier isolates and four deep SSI isolates, partial deletions of the egc cluster were observed. The egc cluster was present in 12 of 13 (92.3%) isolates from deep SSI with persistent infection and in 25 of 41 (61.0%) isolates from patients with successful eradication of their SSI.Z  / Discussion  The MSCRAMM gene bbp was more frequent in deep SSI than in nasal carrier isolates, suggesting a role in S. aureus invasiveness in deep SSI. Bbp belongs to the adhesins, together with fibronectin-binding proteins A and B (FnbpA/B), clumping factors A and B (ClfA/B) and collagen adhesion (Cna). These adhesins have been suggested to mediate S. aureus adhesion on indwelling devices. VW"  0 Discussion   The agr system regulates the production of a diverse range of genes involved in metabolism, virulence, transport and degradation pathways, including the expression of adhesins and the subsequent formation of biofilm . Agr, thereby, plays an important role in S. aureus pathogenesis.V)  1 Discussion   Staphylokinase facilitate bacterial penetration into sur-rounding tissues and is claimed to be an important colonising factor . The presence of sak might be linked to higher invasiveness of S. aureus, finding the gene more frequently in patients than in asymptomatic carriers. @.O8    "  /@8 __WPP6pOp  *"t(  t t 0\Um P   m T*   t 0[m    m d*    t 6Lm _P  m b*    t 6m _  m d*   H t 0޽h ? 3380___PPT10.9 1/r )ea'(  / 0PDArialPrinPP+Bз0зB 0ȷ"D[SOalPrinPP+Bз0зB 0ȷ DWingdingsPP                         ! 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P+Bз0зB 0ȷ0DCalibrisPP+Bз0зB 0ȷ"@DўSOibrisPP+Bз0зB 0ȷ1PD_oŖўrisPP+Bз0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` zr xo      R$tm/"@4b R$&) 茼+dxz R$+?/-INH!>4Rb$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  p# Background  4Staphylococcus aureus is a leading cause of surgical site infections (SSIs) , which are found in more than 50% of infections related to total joint arthroplasty . However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI .Zb  p     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P&   %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.@z      & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) . Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria .L Q  Z    'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '   3 enterotoxins   *:-hemolysin converting phages   +MSCRAMM genes .8     ,Capsule types    2Antibiotic resistance   -Virulence factors   The enterotoxin gene cluster egc (seg, sei, sem, sen, seo, seu),which were found in 47 (82.5%) of the nasal carriers and 41(68.3%) of the SSI isolates. In three nose carrier isolates and four deep SSI isolates, partial deletions of the egc cluster were observed. The egc cluster was present in 12 of 13 (92.3%) isolates from deep SSI with persistent infection and in 25 of 41 (61.0%) isolates from patients with successful eradication of their SSI.Z  / Discussion  The MSCRAMM gene bbp was more frequent in deep SSI than in nasal carrier isolates, suggesting a role in S. aureus invasiveness in deep SSI. Bbp belongs to the adhesins, together with fibronectin-binding proteins A and B (FnbpA/B), clumping factors A and B (ClfA/B) and collagen adhesion (Cna). These adhesins have been suggested to mediate S. aureus adhesion on indwelling devices. VW"  0 Discussion   The agr system regulates the production of a diverse range of genes involved in metabolism, virulence, transport and degradation pathways, including the expression of adhesins and the subsequent formation of biofilm . Agr, thereby, plays an important role in S. aureus pathogenesis.V)  1 Discussion   Staphylokinase facilitate bacterial penetration into sur-rounding tissues and is claimed to be an important colonising factor . The presence of sak might be linked to higher invasiveness of S. aureus, finding the gene more frequently in patients than in asymptomatic carriers. @.O8    "  /@8 __WPP6pOp  3+@(  #  c $L   Molecular characterisation of methicillin-sensitive Staphylococcus aureus from deep surgical site infections in orthopaedic patients    F  C AJC b  < A޽h?"@` 33rT.e0$(  / 0PDArialPrinPP$+=з0зB 0ȷ"D[SOalPrinPP$+=з0зB 0ȷ DWingdingsPP$+=з0зB 0ȷ0DCalibrisPP$+=з0зB 0ȷ"@DўSOibrisPP$+=з0зB 0ȷ1PD_oŖўrisPP$+=з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` zr xk      R$tm/"@4b R$&) 茼+dxz R$+?/-INH!>4Rb$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  # Background  4Staphylococcus aureus is a leading cause of surgical site infections (SSIs) , which are found in more than 50% of infections related to total joint arthroplasty . However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI .Zb  p     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P&   %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.@z      & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) . Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria .L Q  Z    'Results  Clinical data Accessory regulatory gene (agr) groups Clonal complexes Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors Resistance genesP   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '  +MSCRAMM genes .8     ,Capsule types     3 enterotoxins   *:-hemolysin converting phages   2Antibiotic resistance   / Discussion  The MSCRAMM gene bbp was more frequent in deep SSI than in nasal carrier isolates, suggesting a role in S. aureus invasiveness in deep SSI. Bbp belongs to the adhesins, together with fibronectin-binding proteins A and B (FnbpA/B), clumping factors A and B (ClfA/B) and collagen adhesion (Cna). These adhesins have been suggested to mediate S. aureus adhesion on indwelling devices. VW"  0 Discussion   The agr system regulates the production of a diverse range of genes involved in metabolism, virulence, transport and degradation pathways, including the expression of adhesins and the subsequent formation of biofilm . Agr, thereby, plays an important role in S. aureus pathogenesis.V)  1 Discussion   Staphylokinase facilitate bacterial penetration into sur-rounding tissues and is claimed to be an important colonising factor . The presence of sak might be linked to higher invasiveness of S. aureus, finding the gene more frequently in patients than in asymptomatic carriers. @.O8    "  /@8 __WPP6pOp r1/e0V\&(  / 0PDArialPrinPP$+=з0зB 0ȷ"D[SOalPrinPP$+=з0зB 0ȷ DWingdingsPP$+=з0зB 0ȷ0DCalibrisPP$+=з0зB 0ȷ"@DўSOibrisPP$+=з0зB 0ȷ1PD_oŖўrisPP$+=з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` zr xk      R$tm/"@4b R$&) 茼+dxz R$+?/-INH!>4Rb$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  k# Background  4Staphylococcus aureus is a leading cause of surgical site infections (SSIs) , which are found in more than 50% of infections related to total joint arthroplasty . However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI .Zb  p     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P&   %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.@z      & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) . Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria .L Q  Z    'Results  Clinical data Clonal complexes Accessory regulatory gene (agr) groups Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors -hemolysin converting phages Resistance genes*.Z     b %   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '  +MSCRAMM genes .8     ,Capsule types     3 enterotoxins   *:-hemolysin converting phages   2Antibiotic resistance   / Discussion  The MSCRAMM gene bbp was more frequent in deep SSI than in nasal carrier isolates, suggesting a role in S. aureus invasiveness in deep SSI. Bbp belongs to the adhesins, together with fibronectin-binding proteins A and B (FnbpA/B), clumping factors A and B (ClfA/B) and collagen adhesion (Cna). These adhesins have been suggested to mediate S. aureus adhesion on indwelling devices. VW"  0 Discussion   The agr system regulates the production of a diverse range of genes involved in metabolism, virulence, transport and degradation pathways, including the expression of adhesins and the subsequent formation of biofilm . Agr, thereby, plays an important role in S. aureus pathogenesis.V)  1 Discussion   Staphylokinase facilitate bacterial penetration into sur-rounding tissues and is claimed to be an important colonising factor . The presence of sak might be linked to higher invasiveness of S. aureus, finding the gene more frequently in patients than in asymptomatic carriers. @.O8    "  /@8 __WPP6pOp g  ($(  (r ( S Tc&  `}  & r ( S d&  & B (s * ޽h? 33y___PPT10Y+D=' = @B +rJV |'e&V_$(  / 0PDArialPrinPP$+=з0зB 0ȷ"D[SOalPrinPP$+=з0зB 0ȷ DWingdingsPP$+=з0зB 0ȷ0DCalibrisPP$+=з0зB 0ȷ"@DўSOibrisPP$+=з0зB 0ȷ1PD_oŖўrisPP$+=з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` zr xg      R$tm/"@4b R$&) 茼+dxz R$+?/-INH!>4Rb$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  n# Background  4Staphylococcus aureus is a leading cause of surgical site infections (SSIs) , which are found in more than 50% of infections related to total joint arthroplasty . However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI .Zb  p     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P&   %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.@z      & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) . Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria .L Q  Z    'Results  Clinical data Clonal complexes Accessory regulatory gene (agr) groups Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors -hemolysin converting phages Resistance genes*.Z     b %   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '  +MSCRAMM genes .8     ,Capsule types     3 enterotoxins   *:-hemolysin converting phages   2Antibiotic resistance   / Discussion  bThe MSCRAMM gene bbp was more frequent in deep SSI than in nasal carrier isolates, suggesting a role in S. aureus invasiveness in deep SSI. Agr, thereby, plays an important role in S. aureus pathogenesis. The presence of sak might be linked to higher invasiveness of S. aureus, finding the gene more frequently in patients than in asymptomatic carriers.cW).Nb  0 Discussion   BOur study contributes new genotypic information on methicillin-sensitive S. aureus isolates from deep SSI in orthopaedic patients. Further studies to unravel the interface between S. aureus, host infection susceptibility and innate immunity are important for a more extensive understanding of the pathogenesis of SSIs .  BCB  "  /@8 __WPP6pOp   @P*(  Pr P S @Bm  `}  m x P c $Cm , m B Ps * ޽h? 33  PT0(  Tx T c $Qm  `}  m x T c $Rm % m B Ts * ޽h? 33rY 0e5_$(  / 0PDArialPrinPP$+=з0зB 0ȷ"D[SOalPrinPP$+=з0зB 0ȷ DWingdingsPP$+=з0зB 0ȷ0DCalibrisPP$+=з0зB 0ȷ"@DўSOibrisPP$+=з0зB 0ȷ1PD_oŖўrisPP$+=з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` zr xg      R$tm/"@4b R$&) 茼+dxz R$+?/-INH!>4Rb$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g4NdNdB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  n# Background  4Staphylococcus aureus is a leading cause of surgical site infections (SSIs) , which are found in more than 50% of infections related to total joint arthroplasty . However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI .Zb  p     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P&   %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.@z      & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) . Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria .L Q  Z    'Results  Clinical data Clonal complexes Accessory regulatory gene (agr) groups Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors -hemolysin converting phages Resistance genes*.Z     b %   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '  +MSCRAMM genes .8     ,Capsule types     3 enterotoxins   *:-hemolysin converting phages   2Antibiotic resistance   / Discussion  bThe MSCRAMM gene bbp was more frequent in deep SSI than in nasal carrier isolates, suggesting a role in S. aureus invasiveness in deep SSI. Agr, thereby, plays an important role in S. aureus pathogenesis. The presence of sak might be linked to higher invasiveness of S. aureus, finding the gene more frequently in patients than in asymptomatic carriers.cW).Nb  0 Discussion   BOur study contributes new genotypic information on methicillin-sensitive S. aureus isolates from deep SSI in orthopaedic patients. Further studies to unravel the interface between S. aureus, host infection susceptibility and innate immunity are important for a more extensive understanding of the pathogenesis of SSIs .  BCB  "  /@8 __WPP6pOp rާ0eE_$(  / 0PDArialPrinPP$+=з0зB 0ȷ"D[SOalPrinPP$+=з0зB 0ȷ DWingdingsPP$+=з0зB 0ȷ0DCalibrisPP$+=з0зB 0ȷ "@DўSOibrisPP$+=з0зB 0ȷ1PD_oŖўrisPP$+=з0зB 0ȷ"`DArial Unicode MS0зB 0ȷpDHaettenschweiler0зB 0ȷDMistralchweiler0зB 0ȷDLathalchweiler0зB 0ȷ"Deck|ў{SOchweiler0зB 0ȷDTimes New Roman0зB 0ȷDMS PGothicoman0зB 0ȷ"DRockwellcoman0зB 0ȷDeck|[{SOlcoman0зB 0ȷDVerdanacoman0зB 0ȷ"DArial Blackman0зB 0ȷ"DwiSO_GB2312kman0зB 0ȷ DNSe~ў2312kman0зB 0ȷ0DMS UI Gothican0зB 0ȷ"@DNSewiSO Gothican0зB 0ȷPDeckY~{SOothican0зB 0ȷ`DSegoe Printan0зB 0ȷpDSymbolrintan0зB 0ȷDTrebuchet MSan0зB 0ȷ"DSegoe Printan0зB 0ȷDSegoe Printan0зB 0ȷDe[SOe Printan0зB 0ȷ1@ . @n?" dd@  @@`` zr xg      R$tm/"@4b R$&) 茼+dxz R$+?/-INH!>4Rb$|,g!nw>m`{3Eb$4ڬd 6 T%HCb$ cI&;#d ]Sh7b$YGD,qf!Mf*b$ p k^98b$AqRe]Ab*R$˺˾ ;ܒz˧M^dMlR$60,:ӝ 6AA fV+f3ff3̙@83g41d1dB 0ppp@  ʚ;ʚ;<4dddd%F 080___PPT10 pp?  O  n# Background  4Staphylococcus aureus is a leading cause of surgical site infections (SSIs) , which are found in more than 50% of infections related to total joint arthroplasty . However, a recent study showed that nasal S. aureus carriage did not predict S. aureus surgical site infectionsSSI .Zb  p     $ Background  The aim of the study was to identify bacterial molecular markers that distinguish S. aureus strains causing deep SSI from nasal carrier strains in patients undergoing major orthopaedic surgery. In order to cover a large portion of the involved S. aureus genes, a DNA microarray covering approximately 170 bacterial genes and their allelic variants was used to characterise the strains.P&   %Materials and methods  A total of 117 S. aureus isolates obtained from patients undergoing major orthopaedic surgery in 2004-2006 were included. Isolates from 60 patients with deep SSI were collected by swabs from secreting wounds or intraoperative biopsies at soft tissue revision. As controls, 57 nasal S. aureus isolates were collected pre-operatively from patients admitted to elective orthopaedic procedures.@z      & Clinical information(   Patients were categorised into two groups according to the ASA score (<2 and >2) . Deep SSI was defined using the Centers for Disease Control and Prevention (CDC) criteria .L Q  Z    'Results  Clinical data Clonal complexes Accessory regulatory gene (agr) groups Microbial surface components recognising adhesive matrix molecules (MSCRAMM) genes Capsule types Virulence factors -hemolysin converting phages Resistance genes*.Z     b %   ( Clinical data  4Clonal complexes   )&Accessory regulatory gene (agr) groups '  +MSCRAMM genes .8     ,Capsule types     3 enterotoxins   *:-hemolysin converting phages   2Antibiotic resistance   / Discussion  bThe MSCRAMM gene bbp was more frequent in deep SSI than in nasal carrier isolates, suggesting a role in S. aureus invasiveness in deep SSI. Agr, thereby, plays an important role in S. aureus pathogenesis. The presence of sak might be linked to higher invasiveness of S. aureus, finding the gene more frequently in patients than in asymptomatic carriers.cW).Nb  0 Discussion   BOur study contributes new genotypic information on methicillin-sensitive S. aureus isolates from deep SSI in orthopaedic patients. Further studies to unravel the interface between S. aureus, host infection susceptibility and innate immunity are important for a more extensive understanding of the pathogenesis of SSIs .  BCB  "  /@8 __WPP6pOp ry0eUuRoot EntrydO)RSummaryInformation(xUDocumentSummaryInformation8PowerPoint Document(T  /                         ! " # $ % & ' ( ) * + , - . / 0 1 2 3 4 5 6 7 8 9 : ; < = > ? @ A B C D E F G H I J K L M N O P Q R S T U V W X Y Z [ \ ] ^ _ ` a b c d e f g h i j k l m n o p q r s t u v w x y z { } ~  j                   X         ! 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