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The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.ZViral analysisaTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for infl uenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identifi ed as containing infl uenza A on the basis of the M gene, but could not be subtyped.bZb B0 Viral analysisThey were sent to the Chinese National Infl uenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specifi c pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.Z' *x Viral analysis&RNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specifi c real-time RT-PCR or conventional RT-PCR assays for seasonal infl uenza viruses (H1, H3, or B) and avian infl uenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.Z6:?" Viral analysis The full genome of the virus was amplifi ed with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purifi ed from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA), according to the manufacturer s instructions. Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P " ; > ' g  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22) with parameters  -p blastn  e 1e-5  b 10  v 10 after fi ltering of the adapters and human-origin reads. Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identifi ed with MEGAN software (version 4.70.0).16 The percentages of the identifi ed microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes. P ) @c   Viral analysisTo assess the sensitivity of the identifi ed H10N8 virus to neuraminidase inhibitors (oseltamivir and zanamivir), neuraminidase inhibition assays were done as described previously.18 The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells19 separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specifi c serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buff ered saline and tested at an initial dilution of 1/10.CPC!*  @MkVY2 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY63  ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z6} / =Complications, treatment, and clinical outcome of the patient>>$= Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).     0` 33` Sf3f` 33g` f` www3PP` ZXdbmo` \ғ3y`Ӣ` 3f3ff` 3f3FKf` hk]wwwfܹ` ff>>\`Y{ff` R>&- {p_/̴>?" dd@,~?" dd@   " @ ` n?" dd@   @@``PR    @ ` ` p>> (    6  `}  LUSQdkYkHrh7h_    0  `  8USQdkYkHre,g7h_ ,{N~ ,{ N~ ,{V~ ,{N~    0 ^ `  X*  0 ^   Z*  0 ^ `  Z*H  0޽h ? 3380___PPT10.$>Z  ؞!jg$ 0  $(  r  S >  r  S 0F `    H  0޽h ? 3380___PPT10.$`V[$  0 0 $(   r  S \I `}  =  r  S : ` =  H  0޽h ? 3380___PPT10.$pKT$ 0 P$(  r  S H > @  r  S   `     H  0޽h ? 3380___PPT10.$/$  0 @$(  r  S @  `}  @  r  S @  ` @  H  0޽h ? 3380___PPT10.$p E$  0 p$(  r  S 8  `}   r  S p  `   H  0޽h ? 3380___PPT10.$$  0  $(   r  S hC  `}   r  S F  `   H  0޽h ? 3380___PPT10.$V$  0 $$(  $r $ S v  `}   r $ S d}  `   H $ 0޽h ? 3380___PPT10.$U$  0 ($(  (r ( S   `}   r ( S 8(  `   H ( 0޽h ? 3380___PPT10.$@Ϣ$  0 ,$(  ,r , S B  `}   r , S ]  `   H , 0޽h ? 3380___PPT10.$_$  0 4$(  4r 4 S A  `}    r 4 S 9  `   H 4 0޽h ? 3380___PPT10.$'5$  0 0$(  0r 0 S   `}    r 0 S D   `   H 0 0޽h ? 3380___PPT10.$}3F  0 8F(  8r 8 S   `}     8 TA ?@t   H 8 0޽h ? 3380___PPT10.$2$  0 <$(  <r < S t$  `}    r < S ,     H < 0޽h ? 3380___PPT10.$PF  0 @F(  @r @ S   `    @ TA ?V  H @ 0޽h ? 3380___PPT10.$Z;F  0 DF(  Dr D S   `}    D TA ?j'  H D 0޽h ? 3380___PPT10.$erH:DF I5KaMOQSViZ=X\^a]c e 9( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` |D3Oh+'0X4 `h  @ L XdlClinical and epidemiological characteristics of a fatal case of avian infl uenza A H10N8 virus infection : a descriptive studyMichaelMichael39Microsoft Office PowerPoint@w[@PfB$@iEZ*mG2g  i&" WMFC7 DD'lx EMFD'VfZU"F(GDICx!b $$==% % V0xx x % % $$AA" FGDICF(GDICl.wFGDICF(GDIC6ljwFGDICF(GDIC $@FGDICRp@"ArialnUԠUUȲUUp̘iԠUlUUȲUȲUX iWHV A0U00TUh\ԠUȲUih\|UpUdv%    T([$ BU% B'LClinical and epidemiological  % ( Rp@"Arial~U[SOaxxW8[SOU 0 UUWHV A0U00TUUxUU̡E#TiEUpi|ṲUdv%    T(1[$ BU% B0"Lcharacteristics of a fatal case of % ( Rp@"ArialtUԠUUȲUUP̘iԠUlUUȲUȲUX iWHV A0U00TUh\ԠUȲUih\|UpUdv%    T2;[$ BU% B:Lavian influenza A H10N8 virus  % ( Rp@"ArialvU[SOaxxW8[SOU 0 UUWHV A0U00TUUxUU̡E TiEUpi|ṲUdv%    T;D[$ BU% BCLinfection : a descriptive study % ( F(GDIC0kvFGDICRp@"ArialPUԠUUhUU+̘i3ԠUlUUhUhUX i3\>V A0U00TU4h\ԠUhUi4h\|UUdv%    Tx?lJo[$ BU% B?oL\www.the % ( Rp@"ArialPUUhUX i3\>V A0U00TU4h\\>V A0U00U|UUdv+TzQova %p++x,wdv%    TLl[o[$ BU% BLo L`lancet.com % ( Rp@"ArialPUA0U00TU4h\\>V A0U00U|UUdv\>V A0U00ԛU+x,wdv+TzQovb %Dp++x,wdv%    T]lo[$ BU% B]o"LPublished online February 5, 2014  % ( Rp@"ArialIU[SOaxx\>8[SOU 0 UԚU\>V A0U00TUUxUUE0TiEUpi|UV A0U00TUUxUU\>V A0U00U|UV A0U00U|UV A0U00U+x,wdv+TzQove %8p++x,wdv%    Tror[$ BU% Brr Lh6736(14)60111 % ( Rp@"ArialIUA0U00U|UV A0U00U+x,wdv\>V A0U004U+x,wdv+TzQovf %Pp++x,wdv%    TTor[$ BU% BrLP- % ( Rp@"ArialIUA0U00U+x,wdv\>V A0U004U+x,wdv\>V A0U00ԛU+x,wdv+TzQovg %p++x,wdv%    TTo&fWMFCDD'r[$ BU% BrLP2 % ( F(GDIC)O{dFGDICRp@"Arial U[SOaxx P8[SOU 0 U0Ud P\7d A0U00TUUxUUETiEUpi|U(Udv%    T`MUPY[$ BU% BMYLTLi  % ( Rp@"Arial U[SOU 0 U0Ud P\7d A0U00TUUxUUd P\7d A0U00U|U(Udv+TzQovi %"p++x,wdv%    T`QUUY[$ BU% BQYLThui % ( Rp@"ArialUUiṲU(FU4UԠUUԠUUHi(F\ԱUd P\7d A0U00TUDUUL 01UUgiU|ṲUdv%    TIYY][$ BU% BI] L`2014.2.21 % (   x--$xx--'  @@`` |D3     ?2$J /14X'~2$M V/ lᇾ$~2$j1U < Z 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp`5}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$-ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysisaTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for infl uenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identifi ed as containinRoot EntrydO) RW)@PicturesCurrent User,SummaryInformation( 4     A!"#$%&'()*+,-./01234o6789:;<sSBCDQRTUVWXYZ[\]^_`abcdefghijklmn5pZrtuvwxyz|}~      ?2$J /14X'~2$M V/ lᇾ$~2$j1U < Z 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp4}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospita  !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~l in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (Close contacts$<individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review bPowerPoint Document(DocumentSummaryInformation8oard of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.Viral analysisaTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for infl uenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identifi ed as containing infl uenza A on the basis of the M gene, but could not be subtyped.bZb B0 Viral analysisThey were sent to the Chinese National Infl uenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specifi c pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.Z' *x Viral analysis&RNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specifi c real-time RT-PCR or conventional RT-PCR assays for seasonal infl uenza viruses (H1, H3, or B) and avian infl uenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.Z6:?" Viral analysis The full genome of the virus was amplifi ed with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purifi ed from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA), according to the manufacturer s instructions. Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P! ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22) with parameters  -p blastn  e 1e-5  b 10  v 10 after fi ltering of the adapters and human-origin reads. Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identifi ed with MEGAN software (version 4.70.0).16 The percentages of the identifi ed microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes. P ) @c   Viral analysisTo assess the sensitivity of the identifi ed H10N8 virus to neuraminidase inhibitors (oseltamivir and zanamivir), neuraminidase inhibition assays were done as described previously.18 The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells19 separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specifi c serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buff ered saline and tested at an initial dilution of 1/10.CPC!*  @MkVY3 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3  ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 =Complications, treatment, and clinical outcome of the patient>>$ Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).  $ 0  $(  r  S HV>  r  S VF `    H  0޽h ? 3380___PPT10.$`V[$  0 0 $(   r  S @/U `}  U r  S /U ` U H  0޽h ? 3380___PPT10.$pKT$  0 @$(  r  S E `}  E r  S l#E ` E H  0޽h ? 3380___PPT10.$p E$  0 p$(  r  S @3E `}  E r  S  4E ` E H  0޽h ? 3380___PPT10.$r f ١ 1 e] 9( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@@"Arialn-. 32 'Clinical and epidemiological ."System??gkgkg?؇g-@"Arial~-. :2 0"characteristics of a fatal case of.-@"Arialt-. 42 :avian influenza A H10N8 virus .-@"Arialv-. 62 Cinfection : a descriptive study.-@"ArialP-. 0N8 virus infection : a descriptive study IntroductionMethods.Clinical and epidemiological data collection.Clinical and epidemiological data collectionViral analysisViral analysisViral analysisViral analysisViral analysisViral analysisViral analysisResultsYTimeline of the clinical course of the patient and identifi cation of causative pathogen*Table 2 Clinical blood biochemistry testsImaging of the patients chest (ACC) CT scan obtained on day 4. Chest radiographs (patients heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).>Complications, treatment, and clinical outcome of the patientResultsResultsResultsResults6Table 3: Mutations in AH/1 and JX346 viruses, by gene6Table 3: Mutations in AH/1 and JX346 viruses, by geneEpidemiological investigation Discussion Discussion  õg infl uenza A on the basis of the M gene, but could not be subtyped.bZb B0 Viral analysisThey were sent to the Chinese National Infl uenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specifi c pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.Z' *x Viral analysis&RNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specifi c real-time RT-PCR or conventional RT-PCR assays for seasonal infl uenza viruses (H1, H3, or B) and avian infl uenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.Z6:?" Viral analysis The full genome of the virus was amplifi ed with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purifi ed from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA), according to the manufacturer s instructions. Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P! ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22) with parameters  -p blastn  e 1e-5  b 10  v 10 after fi ltering of the adapters and human-origin reads. Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identifi ed with MEGAN software (version 4.70.0).16 The percentages of the identifi ed microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes. P ) @c   Viral analysisTo assess the sensitivity of the identifi ed H10N8 virus to neuraminidase inhibitors (oseltamivir and zanamivir), neuraminidase inhibition assays were done as described previously.18 The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells19 separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specifi c serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buff ered saline and tested at an initial dilution of 1/10.CPC!*  @MkVY3 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3  ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 =Complications, treatment, and clinical outcome of the patient>>$ Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).  $  0 p$(  r  S @3E `}  E r  S  4E ` E H  0޽h ? 3380___PPT10.$r  9( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` D3     ?2$J /14X'~2$M V/ lᇾ$~2$j1U < Z 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp5}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw>   Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysis&RNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specifi c real-time RT-PCR or conventional RT-PCR assays for seasonal infl uenza viruses (H1, H3, or B) and avian infl uenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.Z6:?" Viral analysis The full genome of the virus was amplifi ed with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purifi ed from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA), according to the manufacturer s instructions. Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P! ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22) with parameters  -p blastn  e 1e-5  b 10  v 10 after fi ltering of the adapters and human-origin reads. Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identifi ed with MEGAN software (version 4.70.0).16 The percentages of the identifi ed microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes. P ) @c   Viral analysisTo assess the sensitivity of the identifi ed H10N8 virus to neuraminidase inhibitors (oseltamivir and zanamivir), neuraminidase inhibition assays were done as described previously.18 The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells19 separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specifi c serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buff ered saline and tested at an initial dilution of 1/10.CPC!*  @MkVY3 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3  ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 =Complications, treatment, and clinical outcome of the patient>>$ Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).  $  0 0 $(   r  S @/U `}  U r  S /U ` U H  0޽h ? 3380___PPT10.$pKT$  0  $(   r  S lOE `}  E r  S @@E ` E H  0޽h ? 3380___PPT10.$V$  0 $$(  $r $ S [E `}  E r $ S \E ` E H $ 0޽h ? 3380___PPT10.$Ur  # ?% 9( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` D3     ?2$J /14X'~2$M V/ lᇾ$~2$j1U < Z 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp4}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysis&RNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specifi c real-time RT-PCR or conventional RT-PCR assays for seasonal infl uenza viruses (H1, H3, or B) and avian infl uenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.Z6:?" Viral analysis The full genome of the virus was amplifi ed with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purifi ed from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA), according to the manufacturer s instructions. Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P! ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22) with parameters  -p blastn  e 1e-5  b 10  v 10 after fi ltering of the adapters and human-origin reads. Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identifi ed with MEGAN software (version 4.70.0).16 The percentages of the identifi ed microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes. P ) @c   Viral analysisTo assess the sensitivity of the identifi ed H10N8 virus to neuraminidase inhibitors (oseltamivir and zanamivir), neuraminidase inhibition assays were done as described previously.18 The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells19 separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specifi c serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buff ered saline and tested at an initial dilution of 1/10.CPC!*  @MkVY3 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3  ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 =Complications, treatment, and clinical outcome of the patient>>$ Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).  $  0 $$(  $r $ S [E `}  E r $ S \E ` E H $ 0޽h ? 3380___PPT10.$Ur% &_ c%Ra '2( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` D3     ?2$J /14X'~2$M V/ lᇾ$~2$j1U < Z 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp-}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisTo assess the sensitivity of the identifi ed H10N8 virus to neuraminidase inhibitors (oseltamivir and zanamivir), neuraminidase inhibition assays were done as described previously.18 The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells19 separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specifi c serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buff ered saline and tested at an initial dilution of 1/10.CPC!*  @MkVY3 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3  ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 =Complications, treatment, and clinical outcome of the patient>>$ Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).  $  0 ($(  (r ( S LmE `}  E r ( S mE ` E H ( 0޽h ? 3380___PPT10.$@Ϣ$  0 ,$(  ,r , S {E `}  E r , S |E ` E H , 0޽h ? 3380___PPT10.$_$  0 4$(  4r 4 S đE `}  E r 4 S E ` E H 4 0޽h ? 3380___PPT10.$'5ra    jaA 91( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` H6     ?2$J /14X'~2$M V/ lᇾ$~2$j1U < Z 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp,}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysis  XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3  ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 =Complications, treatment, and clinical outcome of the patient>>$ Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).  $  0 0$(  0r 0 S hE `}  E r 0 S HE ` E H 0 0޽h ? 3380___PPT10.$}3$  0  H$(  Hr H S  `}   r H S < `  H H 0޽h ? 3380___PPT10.U*/ r   e" 2( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@``_useruserhaelnet՜.+,0    nĻʾG' Arial Ĭģ~Clinical and epidemiological characteristics of a fatal case of avian influenza A H1 H6     ?2$J /14X'~2$M V/ lᇾ$~2$j1U < Z 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp-}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC.$c  XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3  ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 =Complications, treatment, and clinical outcome of the patient>>$ Imaging of the patient s chest (A C) CT scan obtained on day 4. 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We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("()  =Complications, treatment, and clinical outcome of the patient>>$ Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).  $  0 <$(  <r < S E `}  E r < S ~E  E H < 0޽h ? 3380___PPT10.$PF  0 0LF(  Lr L S ` `}    L TA ?'g H L 0޽h ? 3380___PPT10.V*1*r[8: 7M= p3( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` L9     O2$J /14X'~2$M V/ lᇾ$~2$j1U < Z2$kk3[ ^ c 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp.}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ r= m= q <( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` T?     O2$J /14X'~2$M V/ lᇾ$~2$j1U < Z2$kk3[ ^ c 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp$7}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian infl uenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species The haemagglutination inhibition antibody titre tested with horse blood cells against JX346 virus was less than 10 for the serum obtained from the patient on day 5, and 80 on day 9 (tZtllF$  0 @P$(  Pr P S  `}   r P S Ԫ `  H P 0޽h ? 3380___PPT10.V*2o$  0 PT$(  Tr T S P `}   r T S i `  H T 0޽h ? 3380___PPT10.V*@o4r=q T q <( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` T?     O2$J /14X'~2$M V/ lᇾ$~2$j1U < Z2$kk3[ ^ c 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp$7}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian infl uenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species The haemagglutination inhibition antibody titre tested with horse blood cells against JX346 virus was less than 10 for the serum obtained from the patient on day 5, and 80 on day 9 (tZtllFr ȱ ;( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` T?^       O2$J /14X'~2$M V/ lᇾ$~2$j1U < Z2$kk3[ ^ c 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp7}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian influenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species. The haemagglutination inhibition antibody titre tested with horse blood cells against JX346 virus was less than 10 for the serum obtained from the patient on day 5, and 80 on day 9 .ttZ$  0 PT$(  Tr T S P `}   r T S i `  H T 0޽h ? 3380___PPT10.V*@o4r7<* h, 9>( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` dK     O2$J /14X'~2$M V/ lᇾ$~2$j1U < Z2$kk3[ ^ c 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp.9}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian influenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species. 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We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian influenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species. The haemagglutination inhibition antibody titre tested with horse blood cells against JX346 virus was less than 10 for the serum obtained from the patient on day 5, and 80 on day 9 .ttHResults Results Results Results F  0 `XF(  Xr X S X `    X TA ?e H X 0޽h ? 3380___PPT10.W*.rsH s >( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @                           ! 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;UuKRc|GWw\"Nalo^c:8/ ^ ]̒92#qq xf*C2:tIB\6k*7E"+vkZr+mϳNvhx bs~yy2?\'+rAv]OHr#!maUWE7=f19m{Ϧ8dD]W `΂Nw:%y8l6InftnCrX^gjF˔rw"6N%ÎDhد\iё׃Tx_H.0]Oe]Բ&F9˅kL:>9Fl[oЕّM?5H%vm|e]?d8ڤ=/8[}"ͺebB lˇP{1[SM1Hu}Lڭj bޚ}DuO[YZ @,7.ICq߈{u_rSCB|e 'Si8KOUwԘ<%O%C*v4 rױ9Crw'gw^ڞ'~S>DjңLL̎ ֓ -H^c49+] MٜYtS{70{Jii*PF-DRVW4ì~iy`#=}B4#2zZ-73>gKViqׁ%\UL"4ԫV?jMqTSRPR 匐|r]HʓtRشJʒeߊkqrڻ>iuEn_\r.sNn]̯U śilܤJJ>[9,ck|yzūۋvX16OھAe ]-8|-u}Cv;xR39zuWІi< \innCXaa᳕ÔcP. s[2 Nsz&66nBxa9%f =[Q\w+żLRk־;]r9 0ra0a0r9 r9 0raS'ˆnD7aPNG  IHDR(sRGB pHYs  g IIDATx^y ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian influenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species. The haemagglutination inhibition antibody titre tested with horse blood cells against JX346 virus was less than 10 for the serum obtained from the patient on day 5, and 80 on day 9 .ttHResults Results Results Results   0 p\F(  \r \ S s `    \ TA ?T H \ 0޽h ? 33___PPT10i.W*&+D=' d= @B +rҴ} $ {?( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@``                         ! 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C=$Y%d_etnȗV|$ M<& ~xPS!ZN95;&9IiVЂWqP(pX*HYGK^-։XE" Q }Saۨ=iIU1#xg/G+pكRvjĈHZ \ȣeܗ삞csx2NpPgȷ($kgl ̅YwƑhCg_WH{Ax+OHGKãa/So֣+.rªg^G>>4'{;ܐxq͙wg0e>$!ʆ]Ks*G\Sx+xa뾬}f)7y|sG-D Kʣ@^pױlӕqyͼ}p\KK놎WA`0x^?^v[E E%EaT%~5G`0 ^:}o/x눯*׏6*ǀG`0 ~9uz,?NG6o_t?<`KvǺ+ͣvo1}+ݱ x%+?V±1#ӱ/g7_Є_zˏ`0D3`c׏;j` ` G`0 `0 <`0xy419@ Z$ϣy] dK     `2$J /14X'~2$M V/ lᇾ$~2$j1U < Z2$kk3[ ^ c 2$L$fڌ[3 2$5KqT gLb$D7a2$]i;(vFEGC0< 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp?  %9}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian influenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species. The haemagglutination inhibition antibody titre tested with horse blood cells against JX346 virus was less than 10 for the serum obtained from the patient on day 5, and 80 on day 9 .ttHResults Results 5Table 3: Mutations in AH/1 and JX346 viruses, by gene"6  - 5   5Table 3: Mutations in AH/1 and JX346 viruses, by gene"6  - 5     0 `F(  `r ` S  `}    ` TA ?(WV H ` 0޽h ? 33___PPT10i.W*+D=' = @B +  0 dF(  dr d S Qd `}  d  d TA ? H d 0޽h ? 33___PPT10i.W*ޞ+D=' = @B +r` 58 <1; G( `/ 0DArial 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` hN     `2$J /14X'~2$M V/ lᇾ$~2$j1U < Z2$kk3[ ^ c 2$L$fڌ[3 2$5KqT gLb$D7a2$]i;(vFEGC0< 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp?  %A}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian influenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species. The haemagglutination inhibition antibody titre tested with horse blood cells against JX346 virus was less than 10 for the serum obtained from the patient on day 5, and 80 on day 9 .ttHResults Results 5Table 3: Mutations in AH/1 and JX346 viruses, by gene"6  - 5   5Table 3: Mutations in AH/1 and JX346 viruses, by gene"6  - 5   Epidemiological investigationThe patient had visited a live poultry market with her carer 4 days befor      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXY^[\]_`abcdefghijklmnopqrstuvwxyz{|}e illness onset. She had bought a chicken after a short stay (about 5 min), but the patient did not handle the chicken (appendix). Additionally, she had no other contact with live poultry or with individuals with fever or influenza-like illness in the 2 weeks before illness onset. She had not travelled anywhere in the previous month.17 close contacts 11 health-care providers, five family members, and one carer were identified. No signs of influenza-like illness were recorded during the investigation period (2 weeks after contact with the patient). Throat swabs obtained from the 11 health-care providers were negative for influenza virus by real-time RT-PCR test. No antibody titre against JX346 virus was detected in the serum samples obtained from the five family members and the carer by haemagglutination inhibition assay._l7 e$  0 h$(  hr h S Td `}  d r h S 0d ` d H h 0޽h ? 3380___PPT10.X*S+rq; M;I  P( `/ 0DArial 0ʾĸģ õƬ0H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` pT     `2$J /14X'~2$M V/ lᇾ$~2$j1U < Z2$kk3[ ^ c 2$L$fڌ[3 2$5KqT gLb$D7a2$]i;(vFEGC0< 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp?  %%J}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysisHRNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3, or B) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.<6ZoZ,:J Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian influenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species. The haemagglutination inhibition antibody titre tested with horse blood cells against JX346 virus was less than 10 for the serum obtained from the patient on day 5, and 80 on day 9 .ttHResults Results 5Table 3: Mutations in AH/1 and JX346 viruses, by gene"6  - 5   5Table 3: Mutations in AH/1 and JX346 viruses, by gene"6  - 5   Epidemiological investigationThe patient had visited a live poultry market with her carer 4 days before illness onset. She had bought a chicken after a short stay (about 5 min), but the patient did not handle the chicken (appendix). Additionally, she had no other contact with live poultry or with individuals with fever or influenza-like illness in the 2 weeks before illness onset. She had not travelled anywhere in the previous month.17 close contacts 11 health-care providers, five family members, and one carer were identified. No signs of influenza-like illness were recorded during the investigation period (2 weeks after contact with the patient). Throat swabs obtained from the 11 health-care providers were negative for influenza virus by real-time RT-PCR test. No antibody titre against JX346 virus was detected in the serum samples obtained from the five family members and the carer by haemagglutination inhibition assay._l7 e Discussion  A novel reassortant avian infl uenza A H10N8 virus was isolated from the tracheal aspirate specimen obtained from the patient 7 days after onset of illness. Sequence analyses revealed that all the genes of the virus were of avian origin, with six internal genes from avian infl uenza A H9N2 viruses. The aminoacid motif GlnSerGly at residues 226 228 of the haemagglutinin protein indicated avian-like receptor binding preference. A mixture of glutamic acid and lysine at residue 627 in PB2 protein which is associated with mammalian adaptation was detected in the original tracheal aspirate samples. ZZZ   H Discussion  The virus was sensitive to neuraminidase inhibitors. Sputum and blood cultures and deep sequencing analysis indicated no co-infection with bacteria or fungi. The first human infection with novel avian influenza A H10N8 virus further increases the importance of surveillance for pandemic preparedness and response. Another human case of infection with H10N8 was reported in Nanchang City on Jan 26, 2014.$  0 l$(  lr l S d `}  d r l S `d ` d H l 0޽h ? 3380___PPT10.Y*@@$  0 p$(  pr p S  ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian influenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species. The haemagglutination inhibition antibody titre tested with horse blood cells against JX346 virus was less than 10 for the serum obtained from the patient on day 5, and 80 on day 9 .ttHResults Results 5Table 3: Mutations in AH/1 and JX346 viruses, by gene"6  - 5   5Table 3: Mutations in AH/1 and JX346 viruses, by gene"6  - 5   Epidemiological investigationThe patient had visited a live poultry market with her carer 4 days before illness onset. She had bought a chicken after a short stay (about 5 min), but the patient did not handle the chicken (appendix). Additionally, she had no other contact with live poultry or with individuals with fever or influenza-like illness in the 2 weeks before illness onset. She had not travelled anywhere in the previous month.17 close contacts 11 health-care providers, five family members, and one carer were identified. No signs of influenza-like illness were recorded during the investigation period (2 weeks after contact with the patient). Throat swabs obtained from the 11 health-care providers were negative for influenza virus by real-time RT-PCR test. No antibody titre against JX346 virus was detected in the serum samples obtained from the five family members and the carer by haemagglutination inhibition assay._l7 e Discussion  A novel reassortant avian infl uenza A H10N8 virus was isolated from the tracheal aspirate specimen obtained from the patient 7 days after onset of illness. Sequence analyses revealed that all the genes of the virus were of avian origin, with six internal genes from avian infl uenza A H9N2 viruses. The aminoacid motif GlnSerGly at residues 226 228 of the haemagglutinin protein indicated avian-like receptor binding preference. A mixture of glutamic acid and lysine at residue 627 in PB2 protein which is associated with mammalian adaptation was detected in the original tracheal aspirate samples. ZZZ   H Discussion  The virus was sensitive to neuraminidase inhibitors. Sputum and blood cultures and deep sequencing analysis indicated no co-infection with bacteria or fungi. The first human infection with novel avian influenza A H10N8 virus further increases the importance of surveillance for pandemic preparedness and response. Another human case of infection with H10N8 was reported in Nanchang City on Jan 26, 2014. 0 xp (  r  S HV>  r  S V,!j    <\7d"`F   bLi hui 2014.2.21 H  0޽h ? 3380___PPT10.$`V[r1E*  U- O( `/ 0DArial2 o?www.the.-@"ArialP-. 2 oL lancet.com.-@"ArialP-. :2 o]"Published online February 5, 2014 .-@"ArialI-. 62 rChttp://dx.doi.org/10.1016/S0140.-@"ArialI-.  2 rq-.-@"ArialI-. 2 rr 6736(14)60111.-@"ArialI-.  2 r-.-@"ArialI-.  2 r2.-@"Arial -.  2 YMLi ..-@"Arial -.  2 YQhui..-@"Arial-. 2 ]I 2014.2.21.- 00H1U2 0"D[SOal 00H1U2 0@ .  @n?" dd@  @@`` pU     `2$J /14X'~2$M V/ lᇾ$~2$j1U < Z2$kk3[ ^ c 2$L$fڌ[3 2$5KqT gLb$D7a2$]i;(vFEGC0< 0AA@3ʚ;ʚ;g4IdId,U2 0ppp@ <4dddd 0$1U 80___PPT10 pp?  %J}Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection : a descriptive study~~$dwww.the lancet.com Published online February 5, 2014 http://dx.doi.org/10.1016/S0140-6736(14)60111-2"eZR$ S Introduction  Human infections with different avian influenza viruses eg, H5N1, H9N2, and H7N9 have raised concerns about pandemic potential worldwide. We report the first human infection with a novel reassortant avian influenza A H10N8 virus.&,9  Methods -Clinical and epidemiological data collection..$Information was obtained about a patient who had been admitted to hospital in Nanchang City, Jiangxi Province, on Nov 30, 2013. epidemiological and clinical data: demographic characteristics; underlying medical conditions; recent exposures to pigs, poultry, or other animals; recent visits to live animal markets; clinical signs and symptoms; chest radiographic findings; clinical laboratory testing results; antiviral treatment; clinical complications; and outcomes. (-Clinical and epidemiological data collection..$ZClose contacts individuals who had provided care to, had been living with, or had potentially been directly exposed to respiratory secretions or bodily fluids of the patient in the 14 days before illness onset were identified. The institutional review board of Nanchang Center for Disease Control and Prevention (CDC) approved the assessment of these close contacts. Written informed consent was obtained from the close contacts.,Viral analysis$vTracheal aspirate specimens were obtained from the patient on day 7 and day 9 after illness onset. Real-time RT-PCR or conventional RT-PCR, or both, were used for influenza typing and subtyping by the Nanchang CDC and Jiangxi provincial CDC. The samples were identified as containing influenza A on the basis of the M gene, but could not be subtyped.$3ZDZw,  Viral analysisThey were sent to the Chinese National Influenza Center on Dec 6, 2013. The tracheal aspirate samples were maintained in a viral-transport medium, and were propagated in the amniotic cavity of 9-day-old specific pathogen-free embryonated chicken eggs for 72 h at 37C. The virus titre was established with a haemagglutination test using turkey red blood cells and was recorded as the reciprocal of the highest dilution of the virus that induced haemagglutination.ZP *x Viral analysis<RNA was extracted from tracheal aspirate samples with QIAamp Viral RNA Mini Kit (Qiagen, Germany), according to the manufacturer s instructions. Specific real-time RT-PCR or conventional RT-PCR assays for seasonal influenza viruses (H1, H3) and avian influenza H1 to H16 and N1 to N9 subtypes were done to verify the viral subtypes. Sequences of the primers and probes are available on request.$6ZiZ>: Viral analysisThe full genome of the virus was amplified with the use of Qiagen OneStep RT-PCR Kit for sequencing(Qiagen, Germany). PCR products were purified from agarose gel with the QIAquick Gel Extraction Kit(Qiagen, Germany). Sequencing was done with the automatic Applied Biosystems 3730xl DNA Analyzer (Life Technologies, USA) and the Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit (LifeTechnologies, USA). Full genome sequences of the viruses were deposited in the Global Initiative on Sharing Avian Influenza Data database (accession number EPI497477-84). A maximum likelihood phylogenetic tree for nucleotide sequences of each gene of selected influenza viruses was constructed with MEGA5.1.P;, ; > ' h  Viral analysisExtracted RNA was reverse transcribed to doublestranded DNA, and deep sequenced on the Ion Torrent platform with the type 318 chip. The resulting sequencing reads were aligned to the National Center for Biotechnology Information non-redundant nucleotide database with BLAST software15 (version 2.2.22). Alignments that had scores greater than 80 were retained for subsequent analysis. The taxonomic composition (at the species level) of each dataset was identified with MEGAN software (version 4.70.0). The percentages of the identified microbial species were estimated according to the numbers of the sequencing reads of each species normalised by their genome sizes.P,)E   Viral analysisPneuraminidase inhibition assays The serum samples obtained from the patient and six close contacts were assessed with the haemagglutination inhibition assay for antibody titre against the H10N8 virus according to standard protocols, with 005% turkey red blood cells and horse red blood cells separately. Before the haemagglutination inhibition assay, serum samples were treated with receptor destroying enzyme (1:4 volume to volume; Denka Seiken, Japan) at 37C for 18 h, and then heat inactivated at 56C for 30 min to remove non-specific serum inhibitors. Serum samples were titrated in two-fold dilutions in phosphate-buffered saline and tested at an initial dilution of 1/10..!PP!b{@KkViral analysisThroat swabs were obtained from the patient s health-care providers and tested for influenza virus by real-time RT-PCR by Nanchang CDC. ResultsThe patient was a woman aged 73 years who had hypertension, coronary heart disease, and myasthenia gravis. She had undergone thymectomy in December,2012. She was reported to have developed initial symptoms of cough and chest tightness on Nov 27, 2013. Fever developed 2 days after illness onset. She was admitted to hospital with fever(3806C) on Nov 30, 2013. A chest CT scan showed consolidation of right lung lower lobe and increased density of left lung lower lobe on day 4. The chest radiograph showed that the patient had bilateral pleural effusion on day 6, with rapid progression of ground-glass opacities and consolidation on day 8 .Z,} 0 XTimeline of the clinical course of the patient and identifi cation of causative pathogenYY,3 )Table 2 Clinical blood biochemistry tests.*("() Imaging of the patient s chest (A C) CT scan obtained on day 4. Chest radiographs (patient s heart on the right) showed mild ground-glass opacities on day 6 (D), and bilateral ground-glass opacities and consolidation on day 8 (E, F).   =Complications, treatment, and clinical outcome of the patient>>$ Resultstracheal aspirate samples obtained from the patient on day 7 and day 9 were shown to be positive for avian influenza A H10N8 virus and negative for seasonal influenza viruses (H1, H3 or B), H5N1, H7N9, and H9N2. Viruses were isolated from the specimens obtained on days 7 and 9 after illness onset, with a haemagglutinin titre of 256 on day 7 and 1024 on day 9. The virus isolated from the sample collected on day 7 was designated as A/Jiangxi-Donghu/346/2013(H10N8) henceforth, JX346 and was used for further analysis. No pathogenic bacteria and fungus were detected in sputum cultures on days 6 and 8, or blood culture on day 6. JZZZ662ResultssThe deep sequencing data from the tracheal aspirate specimen obtained on days 7 and 9 showed that the avian influenza A H10N8 virus was overwhelmingly dominant (>99%) in microbial species. The haemagglutination inhibition antibody titre tested with horse blood cells against JX346 virus was less than 10 for the serum obtained from the patient on day 5, and 80 on day 9 .ttHResults Results 5Table 3: Mutations in AH/1 and JX346 viruses, by gene"6  - 5   5Table 3: Mutations in AH/1 and JX346 viruses, by gene"6  - 5   Epidemiological investigationThe patient had visited a live poultry market with her carer 4 days before illness onset. She had bought a chicken after a short stay (about 5 min), but the patient did not handle the chicken (appendix). Additionally, she had no other contact with live poultry or with individuals with fever or influenza-like illness in the 2 weeks before illness onset. She had not travelled anywhere in the previous month.17 close contacts 11 health-care providers, five family members, and one carer were identified. No signs of influenza-like illness were recorded during the investigation period (2 weeks after contact with the patient). Throat swabs obtained from the 11 health-care providers were negative for influenza virus by real-time RT-PCR test. No antibody titre against JX346 virus was detected in the serum samples obtained from the five family members and the carer by haemagglutination inhibition assay._l7 e Discussion  A novel reassortant avian infl uenza A H10N8 virus was isolated from the tracheal aspirate specimen obtained from the patient 7 days after onset of illness. Sequence analyses revealed that all the genes of the virus were of avian origin, with six internal genes from avian infl uenza A H9N2 viruses. The aminoacid motif GlnSerGly at residues 226 228 of the haemagglutinin protein indicated avian-like receptor binding preference. A mixture of glutamic acid and lysine at residue 627 in PB2 protein which is associated with mammalian adaptation was detected in the original tracheal aspirate samples. ZZZ   H Discussion  The virus was sensitive to neuraminidase inhibitors. Sputum and blood cultures and deep sequencing analysis indicated no co-infection with bacteria or fungi. The first human infection with novel avian influenza A H10N8 virus further increases the importance of surveillance for pandemic preparedness and response. 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