ࡱ> 2;( v/ 0DArialgsRomanTT-ܖ 0ܖ"D[SOalgsRomanTT-ܖ 0ܖ DGaramondRomanTT-ܖ 0ܖ0DTimes New RomanTT-ܖ 0ܖ@DWingdingsRomanTT-ܖ 0ܖPDўSOgdingsRomanTT-ܖ 0ܖ`DTahomagsRomanTT-ܖ 0ܖ"pDGulimgsRomanTT-ܖ 0ܖ"DSymbolgsRomanTT-ܖ 0ܖ A .  @n?" dd@  @@``   8/        !"#$%&'()*+,-./01356 0e0e     A@ A5% 8c8c     ?1 d0u0@Ty2 NP'p<'pA)BCD|E||s " 0e@        @ABC DEEFGHIJK5%LMNOPQRSTUWYZ[ \]^_ `abN E5%  N E5%  N F   5%    !"?N@ABC DEFFGHIJK5%LMNOPQRSTUWYZ[ \]^_ `ab 33@8340K ʚ;ʚ;g4CdCdf_\ N‰[~ހb_`N~ހpe0 2. >e\'`8h }ceQՋ0 ~ހXke DNA Y6R {NW{Qm-NeEQ8hx0(u>e\'`8h }hW{Qm-NcOvz'VvU3HTdR S_DNA Y6Re 3HTdRceQck(WRˆv~ހvDNA-N0~ހXk z^N~ހQceQv3HTdRvϑbckk0Ǐ(umN[ϑKm[W{Q~ހ-Nv>e\'`:_^ 1\SNnx[T~ހXkvRN:_^0 pZ UJJ J3 # ! NˆS[vXkS^ ( [T~ހXkS^vNˆS 1. PHA iir@Q } 2. Con ARFˆ}v A 3. PMN2mFUF ,K8d NˆS[vXkS^ ( b/gel 1. (uRPMI 10\PBMCMb1x106/mlv`m0 2. (u100g/mlvPHAM6R1:1001:20T1:40zʑm0 3. 96T[g-N bNLqQ12T[ kT[-NReQ100l~ހ0c[kv3T[:NN~0MR3~R+RReQNyzʑ^vPHAnm gTN~RW{Qm[gq~ 0 4. 37C 5CO2 54h0 5. M6RSm^:N50ci/mlv3HTdR0 PJGc6 c JJ   NˆS[vXkS^ ( (6. kT[ReQ50ci 3HTdR ~~W{Q18h0 7. (uYT[ꁨR6eƖNR+R6eƖkT[~ހNN\{-N0n~ހ \DNAǏn0Rn~ N mS*gceQv3HTdR gT(u100v2umm N)Rn~r^q0 8. \n~>eeQmꕡ{Q ꁨRpe SbpS~g0 9. cbd,g^ {kN~3*N Y,gvs^GWpe0F J=JI     NˆS[vXkS^ ( q_TV } 1. ~ހ;mRQQb/gq_T~ހ;mR YςT^hg~ހ;mR0 2. W{Qm-NmRv@n gN@nSo;mbb6R~ހXk0Y2kKm[NSO~ހXkR SǑ(ump;mvAB@n0 3. ~ހW{Qg{|W9hnc~ހpeϑǑ(u N Tb_r^萄vW{Qg0 4. _uiralgSMNO~ހXkR0$d- USTmTm]~ހW{QS^ ( \St T~ހSSOYƋ+R Ty_WMHCRP[0S_b2*N N T*NSOvUS*N8h~ހ(WSOYmTe SNvN:Ro _wXk0Xkv:_^N$NKNMHCv]+Rv z^bckk0@bN Ty_WmTm]~ހS^v:_^S f2*N*NSOKNMHC]+Rv z^ v^NV:NvNƋ+RNXk ~g/f2*N*NSOvm]~ހXkRv;`T0ُyՋ1\/fSTmTm]~ހW{QՋ0 \SRS^KNNevUS*N8h~ހ(u>e\'`8h }gq\ OvQ1YSS^R FONOX[:RoR b:N:Ro~ހ RdkevS^~gNS fSNeS^e vXkR0(W[('`hV[y ie SNSm]~ހ[OMHCvS^R @bNTǑ(uUSTmTm]~ހW{QS^0X/Zv  (  USTmTm]~ހW{QՋ ( el 1. Ry[;NNOvPBMC te~ހSm^:N1 x 106/ml0 2. :Ro~ހmp;m(uNˆ }~Sm^25g 37 C 5C 30min b>e\'`8h }gq\2000 rad velYt:Ro~ހ0 3. 96T[g-N kT[ReQ1x105v:Ro~ހT1x105S^~ހ0 37C 5CO2 4^6d0R3HTdR ~~W{Q18h03'`[gqT[RS^~ހTNˆS NR:Ro~ހ04'`[gqT[SRgq\vꁫ~ހ03 Y,g0 4. 6eƖ~ހ mNpe SbpS~g0>PJBJc c 2J Jcckc J M      & USTmTm]~ހW{Q (  5. {v[S^RR [~cpm 4'`[gq~cpm RR 3'`[gq~cpm 4'`[gq~cpm hg USTmTm]~ހW{Q ( lap 1. ~ހ;mRO(uQQ~ހe YςT^hg~ހ;mR0 2. W{Qm-NmRv@n gv@nSO:Robb6RS^0 3. ,g^^\N2000 rpm 3'`[gq~^'YN20000 rpm0,jcd   bS[vyr_'`XkS^ ( NSt ,gՋKm[T~ހ(WSOY[gNyr[bSvyr_'`MQu^T{R0@b(ubSSN/fR!kc扄vbS _NSN/ff~MQuǏvbS08^(uvKm[V_S^vbS g~Sˆ}vMuirPPD T4x$OΘ{|k }TT 0ُ2ybS8^ĉReQRMQucytu bN^[[N gV_S^0(WgNMQu:wu-N [[Nvyr_'`V_S^SQ1_bm1Y0$RH <   bS[vyr_'`XkS^ ( elNT~ހ[4x$OΘ{|k }:RovXkS^:NO 1. RyPBMCTAPC APC(uNˆ }b>e\'`8h }Yt0 2. 96T[g-NkT[ReQ1x105 PBMCT1x104 APC0 3. kT[-NReQ|Rzʑv4x$OΘ{|k }nm N TT[-NbS~Sm^R+R:N00105010T20g/ml0kNySm^3 Y,g0 4. 37C 5CO2 6)Y0 5. R3HTdR ~~W{Q18\e0pe0ZG 2   bS[vyr_'`XkS^ ( ~gʑ 1. [NcyǏ4x$OΘuׂeg ,gՋ~gHTNOS^S f`[4x$OΘ{|k }yr_'`^T{RNO NbhQMQu:w0 2. HIVagS^NO NS fCD4+T~ހQ\[vMQu:w0 D`IJH4  bS[vyr_'`XkS^ ( zlap 1. W{Qm-Ng}Y(uNAB@n0 2. dkT~ހXkS^APC0YO(u~ST~ހ R5^10ꁫAPC0$>:bS[vyr_'`XkS^ ( ^(u[ 1. NˆS[vXkS^ 4N^ N(uNċ0OT~ހ[YKQ:RoBRvXkR S fT~ހW,gMQuR sST~ހ;`SOvOo` NS f*N+RT~ހKQv`Q PHA8^(uNKmՋNT~ހXkR Con A8^(uNKmՋ\ T~ހXkR0433v  bS[vyr_'`XkS^ ( ^(u[ 2. USTmTm]~ހS^ 1 ,gel;N(uN0OhV[y iMROSONSKNMHCvM z^0 2 YW{QeǏ96 \e S6RY~ހk'`T~ހCTL 02a33N  bS[vyr_'`XkS^ ( x^(u[ 3. bS[vyr_'`XkS^ (uN0O:gSO[yr_'`bSv^T{RT:gSO;`SOMQu^T{R0T=33 33'$4B~ހNubSORvKm[ ( W@xt B~ހS0RYKQo;mBRbyr_'`bS:RoT ;mS0Xk gTRSb:NFm~ހ NubSO0bSOSN(W@FmTvQNSOm-NhQ hKmbSO/fKm[B~ހRvg͑vel0 B~ހR{|B1~ހTB2~ހ0$NSuf[ N T0cS:RovbS N T [T~ހvOV'` N T0 1uNB2~ހ[bSv^T{OVT~ހ @bN B~ހNubSORvNO N vQ:w_NSwnNT~ހ:w0&Z@  "NˆS[YKQbSONuRvKm[$ W@xt NB~ހwQ gPWMSSO (WSOYS0RPWM:Roe SuYKQo;m NuYKQbSO0B~ހNubSOvRSN(uELISAKm[W{Q Nn-NbSOvϑ0O _NSN(uELISPOTelKm[NubSOvB~ހpeϑ0O0$ql   "NˆS[YKQbSONuRvKm[$ 0b/gelNċN 1. RyPBMC96T[g-NkT[ReQ1x105~ހTPWM~Sm^1100 04'`[gqT[Q NRpWM0kN[2 Y,g0 2. 37C5CO2W{Q10)YELISA b7)YELISPOT 0 3. 6eƖ Nn (uELISAlKmbSO06eƖ~ހ (uELISPOTlKmNubSOvB~ހ0$P  "NˆS[YKQbSONuRvKm[$ ^(u[ 1. (uNnx[B~ހ;mSTRSvOSl[:g6R NS~ހVP[TvQ[P[[B~ހRSvq_T0 2. V:NB2~ހNubST~ހR @bNSSN(uNhKmT~ހvRR0 3. 4N^ N(uNxvzMQu:wuTꁫMQuu`B~ހRS_8^v:g6R0$}x   "NˆS[YKQbSONuRvKm[$ lap Ǒ(uNbSO:NW@xvb/gYxsl0mmlTAm_~ހN RyvB~ހ QbSO[B~ހNubSOvq_TOۏbb6R\O(u 0,J =.+ ELISPOTl (FW@xt ELISPOThQy:NvTMQuepՋenzyme linked immunospot assay S(uNKm[NuIgGTIgMbSOvB~ހ0vQel/f(ubSSVv 6qTReQbSyr_'`B~ހ0YgB~ހNubSO bSONg NvbS~T0ReQvhNbT>frBR1\O>fr0N*Nep1\NhN*NNubSOv~ހ0 .    3ELISPOTl (b/gel 1. bSS37C2h b4CǏY0Vv}SOS(uZYNps^v0N]xx~~096T[g0 2. bSOub~ހWReQϑbSOub~ހ 37C 5CO2 3^4h bǏY0mm Sd:NNVv~Tv~ހ0 3. Km[epNu~ހRNb 37C 5CO2 2^3h0R9hǏ'lSirvbx'`xxvT^ir>fr0 4 pe24hT(u10^30 P>f_\ Npeepb_b~ހ0 VPPJ4J:r ELISPOTl (:ċN 1. (Wd\O-N^O(uesQbS\O4'`[gqu^cd~ހh,g~bSOalg0 2. ]xx~~ }upOe^ؚNZYNp0 3. NELISATTO(u SN0OUS*N~ހvbSONϑ0 4 S_m]~ހS0R%N͑alge _NSNO(u,gl @bN(uNKm[~~-NbSOub~ހ0 ^(u[ (u TELISAl0,gelS(uNNUOSn'`bS0@P}v /!ELISAKm[T{|MQutˆ}v( W@xt B~ހSbS:RoT gRNuIgMbSO 6qT(WTh~ހNuvTy~ހVP[v\O(u N Sl{|b:NIgG0IgA0IgE{|bSO0^(u N TvUSb ~TELISAel SNKm[B~ހNuvbSOv{|+R0,n `   "ELISAKm[T{|MQutˆ}v(  el 1. Sg96T[g(uSm^:N10g/mlvyr_'`USbS0v Nvg 37C 5CO2 2h b4CǏY08^ĉmg \0 2. N7hkT[QReQ1:10b1:20 zʑv~ހW{Q Nnm100l03'`[gq:NhQT 4'`[gq:NW{Qm0kՋ2 Y,g0[)nW2h b4CǏY0 3. RvhNbkT[R100lx'`xxvhvbNIgMbIgGbIgANb0[)nW2h b4CǏY0 4. >frmg kT[R100l^irp nitrophenyl phosphate 0 5. ꁨRvhNpe NhQf~QbSOSm^0Pc J6% c #cc5         #ELISAKm[T{|MQutˆ}v( HċN ELISAlupOe0{US0_0yr_'`ؚ0͑ Y'`}Y /fKm[ NnTSOm-NIgv el0x'`xxvhvNb>frf>f N_(ux-NbkS^ $\vQ(uNKm[SOYNuvbSO0 ^(u[ ELISASKm[TySOm-NvbSO vQ~gS fh*NSOB~ހR0Km[MQutˆ}vTyN{|v4ls^ gRNt[MQu:wu0<ZCd3 $ CTL@g$ORvKm[ (W@xt CTL:N~ހk'`Tm]~ހv{y0/fCD8 T~ހƋ+RAPChbMHC I{|RP[HTv$vbukyr_'`bST RSb_bv0S_CTLG0Rv^v$v~ހbukag~ހe YǏ~ހc:g6Rbˆ:g6R@g$Ov~ހ0<wJT '%!CTL@g$ORvKm[n1. ~ހc:g6R CTLhFasL v~ހhFas FasLNFasvMc ǏFas OeQvOSo;mv~ހQ~ހQN_ [v~ހ{kN0 2. ~ހˆ:g6R CTLo;mT ހ(Q|T2*N~ހc批pyR0|N~ހTǏYTʑ>e|Q[ir0zT[ }ceQv~ހTZT U1r~ހ Nb_bT[ b~ހˆ0|v[v~ހQN0F33J33_ H&"CTL@g$ORvKm[ (D 51CrhlSt Y(W\v~ހNCTLmTKNMR HQ(u>e\'`8h }51CrW 51CrzǏ~ހۏeQހFm NހFm-N\RP[ˆ}v(brV~T N1uN~ހQʑ>e0S_v~ހCTL4xOWT 51Crʑ>eۏeQ Nn0V:N Nn-N>e\'`:_^N~ހ@g$O z^HTckk ǏKm[ Nn-N>e\'`:_^ 1\SN$R[CTL@g$OR0LJJ5J: +/'#  ($CTL@g$ORvKm[Db/gel 1. N$v~~-NRym]~ހT$v~ހ0 2. 51Crh$v~ހ0 3. 96T[W{QgkT[-NReQm]~ހTv~ހ Hevk:N50:1 25:1 12.5:10Sg'Yʑ>e[gqv~ހRSalBR TSʑ>e[gq NRCTL 0 4. 37C 5CO2 4h0 5. 6eƖ Nn gpehVKm[>e\;m'`cpm 0jJdJ  )%CTL@g$ORvKm[r {lQ_ Ջ~s^GWCPM

e~s^GWcpm

es^GWcpm

es^GWcpm

esؚ vv~ހϑY0O(u>e\'`8h }0P C/33 33 8*&CTL@g$ORvKm[^^(u[ ُNel8^(uNċ0O$v`CTL@g$O$vvR S\O:N$ReTT‰[uHevchKNN0 033++'NK~ހk'`Km[W@xt NK~ހX[(WNYhT@0m]~~-N $\N>g:N0N[0V~ހSOy'YTހFmQ+T'Yϑ| Eey'Y|m]~ހ0hWyr_/fCD16FcgRIII A TCD56^y~~ހ|DRP[ 0S_NK~ހƋ+R$v~ހ;mukag~ހe CD16RP[NT _w1| v^RlIFN gTTNF0|-N+T gzT[ }0|vTproteoglycans _wv~ހn'`{kNTQN0NK~ހvCD16NIgG1TIGg3~T SN[ADCC\O(u0 NK~ހhNyƋ+RHLA A0 B0 CbSvSSO T~ހSQN'`OS ;bkNK~ހv@g$O;m'`0S_v~ހ NhbNOhHLA I{|RP[e b6Rd NK~ހ;mS @g$Ov~ހ0 K562~ހ| NhHLARP[ [NK~ހ@g$O\O(uOea 8^(u\ONK~ހvv~ހ (uNhKmNK~ހv;m'`0 TPH;7       ,(NK~ހk'`Km[db/gel 1. NK~ހvRy(ubCD30CD190CD14USbTmml SdT~ހ0B~ހTMo SNK~ހ0 2. Rg el TCTL@g$OՋ0 N Tv/f v~ހ:NK562~ހ0g'Yʑ>e~:Nv~ހ-NRSalBR Sʑ>e[gq~-N NRNk~ހ037C 5CO2 4h0 3. 6eƖ Nnm gpehVKmTT[cpm>\fg` J*T333` QYmx~3ft` \ғhEy`` cb^DDf`Y˵W` sg7xGr` K%ޯd{mG/` 33f>?" dd@,?nFd@    @ `  n?" dd@   @@``PR    @ ` `<p>> P|(    6L #" `b ` ; X*   6; #" `` ` ; Z* xT ~  "~\ {  "{  c BB CDEFd @ bb   H   T W6Vw}\gFQ6<1++11 1L b6xQrq 6\}N   - c    0 A Q g     S    6N KawF bFy0 a*ly7lE;uz  B | a F 0 ! 1 < B < & l L 0  @`"T   c |BC+DEFyd @ ==gL6cI}\eA& w6m!W<! &<W!r6W}F6\}>68QNlX^XH8+Sgg|@`"Jo 5 J   B! CDEF @ ))\*l{FDNF W% W ! !   ! 6X L b x_ /aF6b}SA$|l\\TX@`s"*  T  u4   B C%DEF ss&QW}k6D Q r 8 N c t y y ;t Qi gS }8     T   :0\-Q4*%%v*o(V}5q;  S   < s } l \ g} \, L ;n &  Z}\[6 }WF<! e C }h m W ]  ,t B RxkqA,Rg@`" {b    BCDEFy @ ,,Lh6\}mWG &a*zCxsh-&jG]~5g6&LLZ\@`s"*   u(     BCDE|Fy @ __@% M|f4@{ f0`lA}L\A+h MB6ww FJl{ 0V|{J  *5KlGn a*:Pf\|6 /6Kbf|F kP}5x}VvaP@@@`s"*   =>    c BlCrDEF"d@ rlrl@`"~  6x; " `} ; LUSQdkYkHrh7h_    6!; #" ``  ; Z*    0,+; " ` ; 8USQdkYkHre,g7h_ ,{N~ ,{ N~ ,{V~ ,{N~  B  s *޽h ? 3___PPT10i. u%.+D=' ;= @B +  Stream  0 `L(  xT ~  "~\ {  "{  c BB CDEFd @ bb   H   T W6Vw}\gFQ6<1++11 1L b6xQrq 6\}N   - c    0 A Q g     S    6N KawF bFy0 a*ly7lE;uz  B | a F 0 ! 1 < B < & l L 0  @`"T   c |BC+DEFyd @ ==gL6cI}\eA& w6m!W<! &<W!r6W}F6\}>68QNlX^XH8+Sgg|@`"Jo 5 J   B! CDEF @ ))\*l{FDNF W% W ! !   ! 6X L b x_ /aF6b}SA$|l\\TX@`s"*  T  u4  B C%DEF ss&QW}k6D Q r 8 N c t y y ;t Qi gS }8     T   :0\-Q4*%%v*o(V}5q;  S   < s } l \ g} \, L ;n &  Z}\[6 }WF<! e C }h m W ]  ,t B RxkqA,Rg@`" {b   BCDEFy @ ,,Lh6\}mWG &a*zCxsh-&jG]~5g6&LLZ\@`s"*   u(     BCDE|Fy @ __@% M|f4@{ f0`lA}L\A+h MB6ww FJl{ 0V|{J  *5KlGn a*:Pf\|6 /6Kbf|F kP}5x}VvaP@@@`s"*   =>    c BlCrDEF"d@ rlrl@`"~  <P5; "F  ; LUSQdkYkHrh7h_    08; " `   ; NUSQdkYkHroRh7h_   6<; #" `` ` ; X*   6G; #" `b  ; Z*   6Q; #" `d ` ; Z* B  s *޽h ? 3___PPT10i. u%.+D=' ;= @B +* 0  *(  x  c $d     r  S    `     H  0޽h ? 3380___PPT10._@fbQ  0 h``(  r  S   `}   r  S 'g    Bw ^ ZMQu~ހRvKm[  H  0޽h ? r3y`f___PPT10i.`09'+D=' ;= @B +  0 p*(  r  S 1  `}   x  c $L2 `  H  0޽h ? r3y`f___PPT10i.`*+D=' ;= @B +  0  *(   r  S E  `}   x  c $pF `  H  0޽h ? r3y`f___PPT10i.`++D=' ;= @B +_  0 vn$(  $r $ S @L  `}   x $ c $H `   $ B[@B bT~ހXkRKm[  H $ 0޽h ? r3y`f___PPT10i.`}$,+D=' ;= @B +u  0 ((  (r ( S Ԛ  `}    ( c $ `  "p`Pp ( BII0 bT~ހXkRKm[  H ( 0޽h ? 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T~ހXkRKm[ ( nW@xt ,gb/g(uNċ0OT~ހ[Ty:RovXkR0T~ހXkR/fS fT~ހRvN*N͑ch0,8-@   T~ހRvKm[ :RoT~ހXkvV }SaIN 1. yr_'`bS _w[KQ;mSXk0 KQ(uN$RebSMQuvHeguׂcy TV_S^R _NS fT~ހ;`SOvR0 2. NˆSYKQ0 3. :RoOSl[RP[YKQ0 Bsdd2  T~ހXkRKm[ ( T~ހXkKm[el 1. >f_\ N‰[~ހb_`N~ހpe0 2. >e\'`8h }ceQՋ0 ~ހXke DNA Y6R {NW{Qm-NeEQ8hx0(u>e\'`8h }hW{Qm-NcOvz'VvU3HTdR S_DNA Y6Re 3HTdRceQck(WRˆv~ހvDNA-N0~ހXk z^N~ހQceQv3HTdRvϑbckk0Ǐ(umN[ϑKm[W{Q~ހ-Nv>e\'`:_^ 1\SNnx[T~ހXkvRN:_^0 pZ UJJ J3 # ! NˆS[vXkS^ ( [T~ހXkS^vNˆS 1. PHA iir@Q } 2. Con ARFˆ}v A 3. PMN2mFUF ,K8d NˆS[vXkS^ ( b/gel 1. (uRPMI 10\PBMCMb1x106/mlv`m0 2. (u100g/mlvPHAM6R1:1001:20T1:40zʑm0 3. 96T[g-N bNLqQ12T[ kT[-NReQ100l~ހ0c[kv3T[:NN~0MR3~R+RReQNyzʑ^vPHAnm gTN~RW{Qm[gq~ 0 4. 37C 5CO2 54h0 5. M6RSm^:N50ci/mlv3HTdR0 PJGc6 c JJ   NˆS[vXkS^ ( (6. kT[ReQ50ci 3HTdR ~~W{Q18h0 7. 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Ry[;NNOvPBMC te~ހSm^:N1 x 106/ml0 2. :Ro~ހmp;m(uNˆ }~Sm^25g 37 C 5C 30min b>e\'`8h }gq\2000 rad velYt:Ro~ހ0 3. 96T[g-N kT[ReQ1x105v:Ro~ހT1x105S^~ހ0 37C 5CO2 4^6d0R3HTdR ~~W{Q18h03'`[gqT[RS^~ހTNˆS NR:Ro~ހ04'`[gqT[SRgq\vꁫ~ހ03 Y,g0 4. 6eƖ~ހ mNpe SbpS~g0>PJBJc c 2J Jcckc J M      & USTmTm]~ހW{Q (  5. {v[S^RR [~cpm 4'`[gq~cpm RR 3'`[gq~cpm 4'`[gq~cpm hg USTmTm]~ހW{Q ( lap 1. ~ހ;mRO(uQQ~ހe YςT^hg~ހ;mR0 2. W{Qm-NmRv@n gv@nSO:Robb6RS^0 3. ,g^^\N2000 rpm 3'`[gq~^'YN20000 rpm0,jcd   bS[vyr_'`XkS^ ( NSt ,gՋKm[T~ހ(WSOY[gNyr[bSvyr_'`MQu^T{R0@b(ubSSN/fR!kc扄vbS _NSN/ff~MQuǏvbS08^(uvKm[V_S^vbS g~Sˆ}vMuirPPD T4x$OΘ{|k }TT 0ُ2ybS8^ĉReQRMQucytu bN^[[N gV_S^0(WgNMQu:wu-N [[Nvyr_'`V_S^SQ1_bm1Y0$RH <   bS[vyr_'`XkS^ ( elNT~ހ[4x$OΘ{|k }:RovXkS^:NO 1. RyPBMCTAPC APC(uNˆ }b>e\'`8h }Yt0 2. 96T[g-NkT[ReQ1x105 PBMCT1x104 APC0 3. kT[-NReQ|Rzʑv4x$OΘ{|k }nm N TT[-NbS~Sm^R+R:N00105010T20g/ml0kNySm^3 Y,g0 4. 37C 5CO2 6)Y0 5. R3HTdR ~~W{Q18\e0pe0ZG 2   bS[vyr_'`XkS^ ( ~gʑ 1. 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RyPBMC96T[g-NkT[ReQ1x105~ހTPWM~Sm^1100 04'`[gqT[Q NRpWM0kN[2 Y,g0 2. 37C5CO2W{Q10)YELISA b7)YELISPOT 0 3. 6eƖ Nn (uELISAlKmbSO06eƖ~ހ (uELISPOTlKmNubSOvB~ހ0$P  "NˆS[YKQbSONuRvKm[$ ^(u[ 1. (uNnx[B~ހ;mSTRSvOSl[:g6R NS~ހVP[TvQ[P[[B~ހRSvq_T0 2. V:NB2~ހNubST~ހR @bNSSN(uNhKmT~ހvRR0 3. 4N^ N(uNxvzMQu:wuTꁫMQuu`B~ހRS_8^v:g6R0$}x   "NˆS[YKQbSONuRvKm[$ lap Ǒ(uNbSO:NW@xvb/gYxsl0mmlTAm_~ހN RyvB~ހ QbSO[B~ހNubSOvq_TOۏbb6R\O(u 0,J =.+ ELISPOTl (FW@xt ELISPOThQy:NvTMQuepՋenzyme linked immunospot assay S(uNKm[NuIgGTIgMbSOvB~ހ0vQel/f(ubSSVv 6qTReQbSyr_'`B~ހ0YgB~ހNubSO bSONg NvbS~T0ReQvhNbT>frBR1\O>fr0N*Nep1\NhN*NNubSOv~ހ0 .    3ELISPOTl (b/gel 1. bSS37C2h b4CǏY0Vv}SOS(uZYNps^v0N]xx~~096T[g0 2. bSOub~ހWReQϑbSOub~ހ 37C 5CO2 3^4h bǏY0mm Sd:NNVv~Tv~ހ0 3. Km[epNu~ހRNb 37C 5CO2 2^3h0R9hǏ'lSirvbx'`xxvT^ir>fr0 4 pe24hT(u10^30 P>f_\ Npeepb_b~ހ0 VPPJ4J:r ELISPOTl (:ċN 1. (Wd\O-N^O(uesQbS\O4'`[gqu^cd~ހh,g~bSOalg0 2. ]xx~~ }upOe^ؚNZYNp0 3. NELISATTO(u SN0OUS*N~ހvbSONϑ0 4 S_m]~ހS0R%N͑alge _NSNO(u,gl @bN(uNKm[~~-NbSOub~ހ0 ^(u[ (u TELISAl0,gelS(uNNUOSn'`bS0@P}v /!ELISAKm[T{|MQutˆ}v( W@xt B~ހSbS:RoT gRNuIgMbSO 6qT(WTh~ހNuvTy~ހVP[v\O(u N Sl{|b:NIgG0IgA0IgE{|bSO0^(u N TvUSb ~TELISAel SNKm[B~ހNuvbSOv{|+R0,n `   "ELISAKm[T{|MQutˆ}v(  el 1. Sg96T[g(uSm^:N10g/mlvyr_'`USbS0v Nvg 37C 5CO2 2h b4CǏY08^ĉmg \0 2. N7hkT[QReQ1:10b1:20 zʑv~ހW{Q Nnm100l03'`[gq:NhQT 4'`[gq:NW{Qm0kՋ2 Y,g0[)nW2h b4CǏY0 3. RvhNbkT[R100lx'`xxvhvbNIgMbIgGbIgANb0[)nW2h b4CǏY0 4. >frmg kT[R100l^irp nitrophenyl phosphate 0 5. ꁨRvhNpe NhQf~QbSOSm^0Pc J6% c #cc5         #ELISAKm[T{|MQutˆ}v( HċN ELISAlupOe0{US0_0yr_'`ؚ0͑ Y'`}Y /fKm[ NnTSOm-NIgv el0x'`xxvhvNb>frf>f N_(ux-NbkS^ $\vQ(uNKm[SOYNuvbSO0 ^(u[ ELISASKm[TySOm-NvbSO vQ~gS fh*NSOB~ހR0Km[MQutˆ}vTyN{|v4ls^ gRNt[MQu:wu0<ZCd3 $ CTL@g$ORvKm[ (W@xt CTL:N~ހk'`Tm]~ހv{y0/fCD8 T~ހƋ+RAPChbMHC I{|RP[HTv$vbukyr_'`bST RSb_bv0S_CTLG0Rv^v$v~ހbukag~ހe YǏ~ހc:g6Rbˆ:g6R@g$Ov~ހ0<wJT '%!CTL@g$ORvKm[n1. ~ހc:g6R CTLhFasL v~ހhFas FasLNFasvMc ǏFas OeQvOSo;mv~ހQ~ހQN_ [v~ހ{kN0 2. ~ހˆ:g6R CTLo;mT ހ(Q|T2*N~ހc批pyR0|N~ހTǏYTʑ>e|Q[ir0zT[ }ceQv~ހTZT U1r~ހ Nb_bT[ b~ހˆ0|v[v~ހQN0F33J33_ H&"CTL@g$ORvKm[ (D 51CrhlSt Y(W\v~ހNCTLmTKNMR HQ(u>e\'`8h }51CrW 51CrzǏ~ހۏeQހFm NހFm-N\RP[ˆ}v(brV~T N1uN~ހQʑ>e0S_v~ހCTL4xOWT 51Crʑ>eۏeQ Nn0V:N Nn-N>e\'`:_^N~ހ@g$O z^HTckk ǏKm[ Nn-N>e\'`:_^ 1\SN$R[CTL@g$OR0LJJ5J: +/'#  ($CTL@g$ORvKm[Db/gel 1. N$v~~-NRym]~ހT$v~ހ0 2. 51Crh$v~ހ0 3. 96T[W{QgkT[-NReQm]~ހTv~ހ Hevk:N50:1 25:1 12.5:10Sg'Yʑ>e[gqv~ހRSalBR TSʑ>e[gq NRCTL 0 4. 37C 5CO2 4h0 5. 6eƖ Nn gpehVKm[>e\;m'`cpm 0jJdJ  )%CTL@g$ORvKm[r {lQ_ Ջ~s^GWCPM

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es^GWcpm

esؚ vv~ހϑY0O(u>e\'`8h }0P C/33 33 8*&CTL@g$ORvKm[^^(u[ ُNel8^(uNċ0O$v`CTL@g$O$vvR S\O:N$ReTT‰[uHevchKNN0 033++'NK~ހk'`Km[W@xt NK~ހX[(WNYhT@0m]~~-N $\N>g:N0N[0V~ހSOy'YTހFmQ+T'Yϑ| Eey'Y|m]~ހ0hWyr_/fCD16FcgRIII A TCD56^y~~ހ|DRP[ 0S_NK~ހƋ+R$v~ހ;mukag~ހe CD16RP[NT _w1| v^RlIFN gTTNF0|-N+T gzT[ }0|vTproteoglycans _wv~ހn'`{kNTQN0NK~ހvCD16NIgG1TIGg3~T SN[ADCC\O(u0 NK~ހhNyƋ+RHLA A0 B0 CbSvSSO T~ހSQN'`OS ;bkNK~ހv@g$O;m'`0S_v~ހ NhbNOhHLA I{|RP[e b6Rd NK~ހ;mS @g$Ov~ހ0 K562~ހ| NhHLARP[ [NK~ހ@g$O\O(uOea 8^(u\ONK~ހvv~ހ (uNhKmNK~ހv;m'`0 TPH;7       ,(NK~ހk'`Km[db/gel 1. NK~ހvRy(ubCD30CD190CD14USbTmml SdT~ހ0B~ހTMo SNK~ހ0 2. Rg el TCTL@g$OՋ0 N Tv/f v~ހ:NK562~ހ0g'Yʑ>e~:Nv~ހ-NRSalBR Sʑ>e[gq~-N NRNk~ހ037C 5CO2 4h0 3. 6eƖ Nnm gpehVKmTT[cpm