Lab Leader: Svetoslav Georgiev Chakarov

Lab Name: Laboratory of Myeloid Cell Biology and Metabolism

Lab Info：Obesity, defined as excess fat mass, increases risks for multiple metabolic diseases, such as type 2 diabetes, cardiovascular disease and several types of cancer. The obesity epidemic has intensified efforts to understand the mechanisms controlling obesity-induced tissue remodeling. Adipose tissue associated macrophages (ATM) are hypothesized to play central roles in these diseases,through their role as inflammatory mediators. In addition to inflammation, a prototypical macrophage function, ATM perform ‘extra’ tissue-specific functions termed accessory functions. One such putative accessory function is their metabolic activity. However, the role of ATM metabolism in shaping tissue remodeling during obesity is poorly understood. In our lab we propose to use a high dimensional flow cytometry-based proteomic screening combining metabolic activities, phenotype and epigenetic, called SCREEN3. This newly developed technic in our lab is easy to use and can be applied for human and mouse samples. In addition, the combination of SCREEN3 with human and mouse single cell RNA sequencing data will allow us to better understand ATM diversity in term of gene expression, phenotype, metabolism and epigenetic remodelling during disease.Finally using Hyperion Imaging Mass Cytometry (IMC) we will validate and characterize the crosstalk between ATM and their adipose tissue microenvironment

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