Research Field

Our lab is mainly interested in the function and underlying mechanisms of organelle stress in disease occurrence and tissue regeneration. The well-established research systems include mouse and human hematopoietic stem cell maintenance, leukemia stem cell and leukemogenesis. We focus on the following scientific questions: How mitochondrial stress is involved in hematopoietic regeneration and leukemogenesis? What’s the relation between ER stress and cell fate determination of hematopoietic stem cells? Does nucleolus stress response pathway play some role in hematopoietic stem cell homeostasis? Screening for small molecule drugs of human hematopoietic stem cell ex vivo expansion. Our studies will facilitate the development of efficient approaches to treat malignant or non-malignant blood diseases.

Personal Introduction

EDUCATION:

• 09/2011-07/2014     Ph.D., Biochemistry and Molecular Biology, National Institute                of Biological Sciences (NIBS) & China Agricultural University (CAU), Beijing, China.

• 09/2007-07/2010    M.S., Microbial & Biochemical Pharmacy, Beijing Normal University (BNU), Beijing, China.

• 09/2003-06/2007     B.S., Biotechnology, Central-South University of Forestry and Technology (CSUFT), Changsha, China.

RESEARCH EXPERIENCE:

• 12/2018-present     Principal Investigator, Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

• 03/2015-11/2018     Postdoctoral Fellow, Dr. Hal E. Broxmeyer’ Lab, Indiana University School of Medicine, Indianapolis, Indiana, USA.

• 07/2014-12/2014      Postdoctoral Fellow, Dr. Hong Zhang’ Lab, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

• 07/2010-08/2011     Technician, Dr. Hong Zhang’ Lab, National Institute of Biological Sciences, Beijing, China.

Scientific Research Projects

• 09/2019-12/2023     National Key Research and Development Project/973 Program (2019YFA0111800) PI

• 01/2020-12/2022     Major Research Plan of National Natural Science Foundation of China (91957107) PI

• 01/2020-12/2023     General Program of National Natural Science Foundation of China (81970095) PI

Scientific Research Projects

• 09/2019-12/2023     National Key Research and Development Project/973 Program (2019YFA0111800) PI

• 01/2020-12/2022     Major Research Plan of National Natural Science Foundation of China (91957107) PI

• 01/2020-12/2023     General Program of National Natural Science Foundation of China (81970095) PI

Publications

• Jin Y*, Wang Q*, Ding Q*, Yao C, Jiang R#, Guo B#, Meng Q#. H6PD overexpression promotes ex vivo expansion of human cord blood hematopoietic stem cells. Stem Cell Reviews and Reports. (2022). https://doi.org/10.1007/s12015-022-10352-w (#Corresponding author)

• Chen Y*, Fang S*, Ding Q*, Jiang R*, He J, Wang Q, Jin Y, Huang X, Liu S, Capitano ML, Trinh T., Teng Y#, Meng Q#, Wan J#, Broxmeyer HE#, and Guo B#. (2021). ADGRG1 enriches for functional human hematopoietic stem cells following ex vivo expansion-induced mitochondrial oxidative stress. J. Clin. Invest. 131(20):e148329. doi: 10.1172/JCI148329. (#Corresponding author)

• Jin Y*, Huang J*, Wang Q, He J, Teng Y, Jiang R#, Broxmeyer HE#, Guo B#. RXR Negatively Regulates Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem and Progenitor Cells. (2021). Stem Cell Reviews and Reports. 17(4):1456-1464. https://doi.org/10.1007/s12015-021-10124-y (#Corresponding author)

• Chen Y*, Yao C*, Teng Y, Jiang R, Huang X, Liu S, Wan J, Broxmeyer HE#, Guo B#. (2019). Phorbol ester induced ex vivo expansion of rigorously-defined phenotypic but not functional human cord blood hematopoietic stem cells: a cautionary tale demonstrating that phenotype does not always recapitulate stem cell function Leukemia 33:2962-2966. (#Corresponding author)

• Huang X*, Guo B*, Liu S, Wan J, Broxmeyer HE. (2018). Neutralizing negative epigenetic regulation by HDAC5 enhances human hematopoietic stem cell homing and engraftment. Nature Communications 9:2741. (*Co-first author)

• Guo B*, Huang X*, Lee MR, Lee SA, Broxmeyer HE. (2018). Antagonism of PPAR-γ signaling expands human hematopoietic stem and progenitor cells by enhancing glycolysis. Nature Medicine 24:360-367. (*Co-first author)

• Guo B, Huang X, Cooper S, Broxmeyer HE. (2017). Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment. Nature Medicine 23:424-428 (Top Story of Cell Therapy News 18.07 March 6, 2017; Top Story of Cord Blood News 9.09 March 9, 2017).

• Guo B*, Liang Q*, Li L, Hu Z, Wu F, Zhang P, Ma Y, Zhao B, Kovas AL, Zhang Z, Feng D, Chen S, Zhang H. (2014). O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Nature Cell Biology 16:1215-1226. (*Co-first author)

• Guo B*, Huang X*, Zhang P, Qi L, Liang Q, Zhang X, Huang J, Fang B, Hou  W, Han J, Zhang H. (2014). Genome-wide screen identifies signaling pathways that regulate autophagy during C. elegans development. EMBO reports 15:1001-1102. (*Co-first author)

• Guo B*, Huang J*, Wu W, Feng D, Wang X, Chen Y, Zhang H. (2014). The nascent polypeptide-associated complex is essential for autophagic flux. Autophagy 10:1738-1748. (*Co-first author)

• Zhang H, Chang J, Guo B, Hansen M, Jia K, Kovacs AL, Kumsta C, Lapierre LR, Legouis R, Lin L, Lu Q, Melendez A, O’Rourke AJ, Sato K, Sato M, Wang X, Wu F. (2015). Guidelines for monitoring autophagy in C. elegans. Autophagy. 11:9-27.

• Lu Q, Yang P, Huang X, Hu W, Guo B, Wu F, Lin L, Kovács AL, Yu L, Zhang H. (2011). The WD40 repeat PtdIns(3)P-binding protein EPG-6 regulates progression of omegasomes to autophagosomes. Developmental Cell 21: 343-357.