生物传感与环境健康课题组

Biosensing and Environmental Health

一、       PI介绍

Principal investigator

       

殷堃，研究员，上海市海外高层次人才。2016年博士毕业于中国科学院大学。2016-2021年分别在俄亥俄州立大学、宾夕法尼亚大学与康尼狄格大学担任博士后研究员。主持了一系列新型、快速、超灵敏、高通量分析诊断技术及传染性疾病移动互联智能检测平台的开发研究。目前已发表SCI论文、IEEE会议论文30余篇，其中在Chemical Engineering Journal（IF = 13.273）、Theranostics（IF = 11.556）、Water Research（IF = 11.236）、Biosensors and Bioelectronics（IF = 10.618）、Analytical Chemistry（IF = 6.986）、Lab on a Chip（IF = 6.799）中科院一区期刊上以第一作者身份发表SCI论文11篇，影响因子10以上论文6篇，被他引1000余次。此外以通讯作者出版学术专著2部，获授权中国发明专利8项。曾获得“中国科学院院长特别奖”、“中国科学院优秀博士生论文”等荣誉。

    Professor Kun Yin obtained a Ph.D. degree from the University of Chinese Academy of Sciences in 2016 and completed a postdoctoral fellowship at Ohio State University, University of Pennsylvania and University of Connecticut, respectively between 2016-2021. His research interests focus on the development of novel, fast, ultra-sensitive, high-throughput analysis and diagnosis technologies and mobile Internet intelligent detection platform for infectious disease diagnosis. He has published more than 30 SCI papers and IEEE conference papers in total. Prof. Yin has published 11 SCI papers including Chemical Engineering Journal (IF = 13.273), Theranostics (IF = 11.556), Water Research (IF = 11.236), Biosensors and Sensors, Analytical Chemistry (IF = 6.986), and Lab on a Chip (IF = 6.799) as the first author. In addition, Prof. Yin has published two books as the corresponding author and has been authorized 8 Chinese invention patents. He has selected as "Special Award of President of Chinese Academy of Sciences" and "Excellent Doctoral Thesis of Chinese Academy of Sciences".

 

二、       研究组介绍

Introduction

生物传感与环境健康课题组主要在人类与环境界面上进行生物安全风险防控与治理体系建设，提高国家生物安全治理能力，切实保护国民生命健康安全。主要的研究方向为病原微生物检测、抗生素耐药防控等。目前，环境健康与生态安全系承担国家级、部委级、校级等多项研究项目，在研经费近300万。研究组成员包括：胡沁沁、修乐山。

    Biosensing and Environmental Health research group focuses on biosafety risk prevention, control and management system construction at the human-environment interface, improves national biosafety management capacity, and effectively protects health safety of citizens. The research mainly focus on infectious disease diagnosis, antibiotic resistance prevention, etc. At present, the Department of Environmental Health and Ecological Security is in charge of a number of national, ministerial, university-level research projects, with nearly 3 million research funds. Members include Qin-qin Hu and Le-shan Xiu.

 

三、       代表性科研成果介绍

Major Research Achievements of the Department

（一）  项目资助情况

Project funding

1.     国家自然科学基金青年项目：基于CRISPR/Cas识别机理的新型冠状病毒多靶位可视化诊断方法研究（22104090）

Novel Coronavirus Multi-target visual diagnosis Method based on CRISPR/Cas recognition mechanism, 22104090, Youth Foundation of National Natural Science Foundation of China.

2.     转化医学国家重大科技基础设施（上海）开放课题项目：级联放大-适配体传感器用于诊断耐药型TNBC外泌体（TMSK-2021-114）

Aptasensors with cascades amplification for diagnosis of TNBC exosomes, TMSK-2021-114, National Facility of Translational Medicine (Shanghai).

3.     崇明岛全健康先导项目研究：崇明岛人-动物-环境界面的废水耐药性监测与控制（SGHKJCX20221-02）

Monitoring and control of wastewater resistance at human-animal-environment interface in Chongming Island, SGHKJCX20221-02, One Health Pilot Study on Chongming Island's.

4.     国家卫生健康委寄生虫病原与媒介生物学重点实验室开放课题基金：基于环境核酸的血吸虫现场监测预警技术研究：（NHCKFKT2021-06）

Research on on-site monitoring and early warning technology of Schistosoma japonicum based on environmental nucleic acid, NHCKFKT2021-06, Open Project Fund of Key Laboratory of Parasite And Vector Biology, NHC.

 

（二）  代表性论文

Selected Publications

1.       Chen Chen, Qinqin Hu, Kun Yin*. Visible transparency tuning and corresponding sensing application of opal photonic crystals. Optics Express, 2021. (JCR 1区)

2.       Kun Yin, Xiong Ding, Zhiheng Xu, Ziyue Li, Xingyu Wang, Hui Zhao, Clifford Otis, Baikun Li, Changchun Liu*. Multiplexed colorimetric detection of SARS-CoV-2 and other pathogens in wastewater on a 3D printed integrated microfluidic chip. Sensors and Actuators B: Chemical, 2021, 130242. (IF=7.460)

3.       Kun Yin, Xiong Ding, Ziyue Li, Maroun M. Sfeir, Enrique Ballesteros, Changchun Liu*. Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2. Lab on a Chip, 2021, (IF=6.799)

4.       Kun Yin, Xiong Ding, Ziyue Li, Hui Zhao, Kumarasen Cooper, Changchun Liu*. Dynamic aqueous multiphase reaction system for one-pot CRISPR-Cas12a based ultrasensitive and quantitative molecular diagnosis. Analytical Chemistry, 2020, 92:8561-8568. (IF= 6.986)

5.       Kun Yin, Vikram Pandian, Karteek Kadimisetty, Xin Zhang, Carlos Ruiz, Kumarasen Cooper, Changchun Liu*. Real-time colorimetric Quantitative Molecular Detection of infectious Diseases on Smartphone-based Diagnostic platform. Scientific Reports, 2020, 10：1-9. (IF= 4.379)

6.       Kun Yin, Vikram Pandian, Karteek Kadimisetty, Carlos Ruiz, Kumarasen Cooper, Jianxin You, and Changchun Liu*. Synergistically enhanced colorimetric molecular detection using smart cup: a case for instrument-free HPV-associated cancer screening. Theranostics, 2019, 9: 2637-2645. (IF= 11.556)

7.       Kun Yin, Qiaoning Wang, Min Lv, Lingxin Chen*. Microorganism remediation strategies towards heavy metals. Chemical Engineering Journal, 2019, 360: 1553-1563. (IF= 13.273)

8.       Kun Yin, Min Lv, Qiaoning Wang; Yixuan Wu; Chunyang Liao*, Lingxin Chen. Simultaneous Bioremediation and Biodetection of Mercury Ion through Surface Display of Carboxylesterase E2 from Pseudomonas Aeruginosa PA1. Water Research, 2016. 103: 383-390. (IF= 11.236)

9.       Kun Yin, Yixuan Wu, Shasha Wang and Lingxin Chen*. A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine. Sensors and Actuators B: Chemical, 2016, 232: 257-263. (IF=7.460)

10.    Kun Yin, Fabiao Yu, Dongyan Liu, Zhihong Xie and Lingxin Chen*. Cyanine-based colorimetric and fluorescent probe for the selective detection of diethylstilbestrol in seawater, shrimp and fish samples. Sensors and Actuators B: Chemical, 2016, 223: 799-805. (IF=7.460)

11.    Kun Yin, Fabiao Yu*, Weiwei Zhang, and Lingxin Chen*. A near-infrared ratiometric fluorescent probe for cysteine detection over glutathione indicating mitochondrial oxidative stress in vivo. Biosensors and Bioelectronics, 2015, 74: 156-164. (IF=10.618)

12.    Kun Yin, Bowei Li, Xiaochun Wang, Weiwei Zhang* and Lingxin Chen*. Ultrasensitive colorimetric detection of Cu²⁺ ion based on catalyticoxidation of L-cysteine. Biosensors and Bioelectronics, 2015, 64: 81-87. (IF=10.618)

13.    Kun Yin, Weiwei Zhang and Lingxin Chen*. Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone. Biosensors and Bioelectronics, 2014, 51: 90-96. (IF=10.618)

14.    Kun Yin, Leming Sun, Zhen Fan, Huacan Lin, Yujian Huang, Scott Galster, Jeff, Kuret, Douglas Scharre, Mingjun Zhang*. Fluorescent peptide nanoparticles to monitor Aβ oligomers. IEEE-NANO, 2017 IEEE 17th International Conference on Nanotechnology, 2017, 25-28.

15.    Zhiheng Xu, Kun Yin, Xiong Ding, Ziyue Li, Xuanhao Sun, Baikun Li, Rajesh V. Lalla, Robert Gross, and Changchun Liu.An integrated E-Tube cap for sample preparation, isothermal amplification and label-free electrochemical detection of DNA. Biosensors and Bioelectronics. 2021,186:113306. (IF=10.618)

16.    Xiong Ding, Kun Yin, Ziyue Li, Maroun M. Sfeir, and Changchun Liu. Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay. Biosensors and Bioelectronics. 2021, 184: 113218. (IF=10.618)

17.    Karteek Kadimisetty, Kun Yin, Aoife M. Roche, Yanjie Yi, Frederic D. Bushman, Ronald G. Collman, Robert Gross, Liang Feng, and Changchun Liu. "An integrated self-powered 3D printed sample concentrator for highly sensitive molecular detection of HIV in whole blood at the point of care." Analyst 2021, 146: 3234-3241. (IF=3.978)

18.    Xiong Ding, Kun Yin, Ziyue Li, Rajesh V. Lalla, Enrique Ballesteros, Maroun M. Sfeir, Changchun Liu*. Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay. Nature Communications, 2020, 11:1-10. (IF=11.800)

19.    Weiwei Zhang, Kun Yin, Bowei Li and Lingxin Chen*. A glutathione S-transferase from Proteus mirabilis involved in heavy metal resistance and its potential application in removal of Hg²⁺. Journal of Hazardous Materials, 2013, 261: 646-652. (IF=9.038)

20.    Weiwei Zhang, Kun Yin and Lingxin Chen*. Bacteria-mediated bisphenol A degradation. Applied Microbiology and Biotechnology, 2013, 97: 5681-5689. (IF=3.670)

21.    Huacan Lin, Kun Yin, Zhen Fan, Jianjie ma, Mingjun Zhang*. Integrating tea nanoparticle with short peptides to generate tunable hydrogel scaffolds for diabetic ulcer. IEEE-NANO, 2017 IEEE 17th International Conference on Nanotechnology

22.    Xiong Ding, Kun Yin, Ju Chen, Kepeng Wang, Changchun Liu*. A ribonuclease-dependent cleavable beacon primer triggering DNA amplification for single nucleotide mutation detection with ultrahigh sensitivity and selectivity. Chemical Communications. 2019;55(84):12623-6. (IF=6.164)

 

（三）  发明专利

Patents

1.     陈令新, 殷堃, 于法标, 陈浩, 张卫卫. 一种基于花菁的有机化合物及其应用（专利号: CN104119263B）

2.     陈令新, 殷堃, 于法标, 张卫卫, 陈浩, 刘萍. 基于花菁的有机化合物及其应用（专利号: CN104098500A）

3.     陈令新, 殷堃, 张卫卫. 一种简便快速检测L-半胱氨酸的方法（专利号: CN103969253A）

4.     陈令新, 殷堃, 张卫卫. 一种简便快速检测Cu²⁺的方法（专利号: CN103969254B）

5.     陈令新, 殷堃, 张卫卫. 一种生物元件用于检测痕量二价铜离子的方法及其应用（专利号：CN104819972A）

6.     陈令新, 殷堃, 于法标. 一种基于花菁的探针用于检测痕量二价铜离子的方法及其应用（专利号：CN104849271B）

 

（四）  获奖及各级人才情况

Awards and talents at all levels

1.     2021，上海市海外高层次人才（殷堃）

Kun Yin, Shanghai overseas high-level talents, 2021.