Min Liu, M.S  

Mail: minliu1265@gmail.com

Education:

Sep. 2005–Jul. 2009  Bachelor of Science   

Chengdu University of Technology, Chengdu, China

Major: Bioengineering

Sep. 2009–Jul. 2012   M.S. in Botany

Research Center for Plant Ecology, Chinese Academy of Sciences , China

Advisors: Professor Xia bing

Major: botany

Work Experience:

July. 2012-Mar. 2016  Research Assistant

                   Interdisciplinary Research Center on Biology and Chemistry,

                   Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences

                   Shanghai, China

Advisor: Dr. Junying Yuan

Aug. 2016- Aug.2018  Research Assistant II

                   Boston Children’s Hospital, Harvard  Medical School

                   Advisor: Dr. Leslie Silberstein

Aug. 2018- May.2019  Research Assistant III

                   Boston Children’s Hospital, Harvard  Medical School

                     Advisor: Dr. Leslie Silberstein

May. 2019-Sep. 2021   Research Technologist

                    Boston Children’s Hospital, Harvard  Medical School

                    Advisor: Dr. Leslie Silberstein

Sep.2021-Apr.2023     Lab Research Supervisor

Boston Children’s Hospital, Harvard  Medical School

                    Advisor: Dr. Min Dong

May. 2023-Present      Research Associate

                    Shanghai Jiao Tong University School Of Medicine Songjiang Institute

Research Direction：

The function and mechanisms of cell death and inflammation in human diseases

Key experimental skills and methods

● Cell culture, Isolation and culture of primary cells from mice different organs

● Isolation and analysis of DNA, RNA, and protein from cultured cells and animal tissues (qPCR, RT-PCR, Western Blot, ELISA)

● CRISPR/Cas-mediated knock-out cell line generation, lentivirus-mediated shRNA delivery, Plasmid construction)

● Protein purification and enzyme digestion reaction

● Analysis of protein modifications (Kinase-Phosphatase assay, Ubiquitination-Deubiquitination assay)

● Experiments for confirming protein-protein interaction (Co-Immunoprecipitation (Co-IP), GST-Pull down assay)

● Animal experiments (Genotyping, Intraperitoneal Injection, Intravenous injection and Subcutaneous injection, Bone marrow transplantation)

● Microtome Sectioning, Immunohistochemistry.

● Flow cytometer

Peer-Reviewed Publications:

1.Manion J, Musser MA, Kuziel GA, Liu M (刘敏，共同第二作者), Shepherd A, Wang S, Lee PG, Zhao L, Zhang J, Marreddy RKR, Goldsmith JD, Yuan K, Hurdle JG, Gerhard R, Jin R, Rakoff-Nahoum S, Rao M, Dong M. C. difficile intoxicates neurons and pericytes to drive neurogenic inflammation. Nature. 2023 Sep 12. doi: 10.1038/s41586-023-06607-2. 

2.Zhang T#, Xu D#,*, Trefts E#, Lv M#, Inuzuka H, Song G, Liu M (刘敏，第四作者), Lu J, Liu J, Chu C, Wang M, Wang H, Meng H, Liu H, Zhuang Y, Xie X, Dang F, Guan D, Men Y, Jiang S, Jiang C, Dai X, Liu J, Wang Z, Yan P, Wang J, Tu Z, Babuta M, Erickson E, Hillis AL, Dibble CC, Asara JM, Szabo G, Sicinski P, Miao J, Lee YR, Pan L*, Shaw RJ*, Yuan J*, Wei W*. Metabolic orchestration of cell death by AMPK-mediated phosphorylation of RIPK1. Science. 2023 Jun 30;380(6652):1372-1380.

3.Zhang T#, Xu D#,*, Liu J#, Wang M#, Duan LJ, Liu M (刘敏，第三作者), Meng H, Zhuang Y, Wang H, Wang Y, Lv M, Zhang Z, Hu J, Shi L, Guo R, Xie X, Liu H, Erickson E, Wang Y, Yu W, Dang F, Guan D, Jiang C, Dai X, Inuzuka H, Yan P, Wang J, Babuta M, Lian G, Tu Z, Miao J, Szabo G, Fong GH, Karnoub AE, Lee YR, Pan L, Kaelin WG Jr, Yuan J*, Wei W*. Prolonged hypoxia alleviates prolyl hydroxylation-mediated suppression of RIPK1 to promote necroptosis and inflammation. Nature Cell Biology. 2023 Jul;25(7):950-962. 

4.Tian S, Xiong X, Zeng J, Wang S, Tremblay BJ, Chen P, Chen B, Liu M (刘敏), Chen P, Sheng K, Zeve D, Qi W, Breault DT, Rodríguez C, Gerhard R, Jin R, Doxey AC, Dong M. Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants. Nature Communications. 2022 Nov 9;13(1):6786.

5.Park S, Matte A, Jung Y, Ryu J, Anand W, Han E, Liu M (刘敏), Carbone C, Melisi D, Nagasawa T, Locascio J, Lin C, Silberstein L, Franceschi L. Pathologic angiogenesis in the bone marrow of humanized sickle cell mice is reversed by blood transfusion. Blood. 2020 Jun 4;135(23):2071-2084

6.Zhang T, Dong K, Liang W, Xu D, Xia H, Geng J, Najafov A, Liu M (刘敏), Li Y, Han X, Xiao J, Jin Z, Peng T, Gao Y, Cai Y, Qi C, Zhang Q, Sun A, Lipinski M, Zhu H, Xiong Y, Pandolfi P, Li H, Yu Q and Yuan J. G-protein Coupled Receptors Regulate Autophagy by ZBTB16-mediated Ubiquitination and Proteasomal Degradation of Adaptor Protein Atg14L. eLIFE, 2015

7.Xu D, Shan B#, Lee B, Zhu K, Zhang T#, Sun H, Liu M (刘敏), Shi L, Liang W, Qian L, Xiao J, Wang L, Pan L, Finley D, Yuan J. Phosphorylation and activation of ubiquitin-specific protease-14 by Akt regulates the ubiquitin-proteasome system. eLIFE, 2015.

8.Xiao J, Zhang T, Xu D, Wang H, Cai Y, Jin T, Liu M (刘敏), Liu M, Jin M, Wu K, Yuan J. FBXL20-mediated Vps34 ubiquitination as a p53 controlled checkpoint in regulating autophagy and receptor degradation. Genes Development. 2015 Jan 15;29(2):184-96.

#: These authors contribute equally to this work.

*:  Corresponding author